Priyanka

A Stability indicating isocratic liquid chromatographic method with UV detection at 255 nm is described for simultaneous determination of sofosbuvir and velpatasvir  in its bulk and tablet dosage form. Chromatographic separation of two drugs was achieved on a YMC column (4.6×15mm,5 ) using a mobile phase consisting of a binary mixture of acetonitrile and 0.025M KH2PO4  adjusted to pH3..0 with orthophosphoric acid in ratio 50:50. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. Linearity, accuracy and precision were found to be acceptable over the concentration range of 50-250 µg/ml for velpatasvir and 200-1000µg/ml for sofosbuvir and R2 found to be 0.999. Accuracy was measured via recovery studies and found to be acceptable, and the percentage recoveries were found in the range of 97-103%.Method precision results obtained are 0.1%RSD for sofosbuvir and 0.8%RSD for velpatasvir. Forced degradation studies were also conducted, and the drugs were subjected to various stress conditions such as acid hydrolysis, base hydrolysis, oxidative, photolytic and thermal degradation. The proposed method was successfully validated and applied for the quantitative estimation of these drugs in both bulk and tablet dosage forms. The LC method can be used for the quality control of formulated products containing sofosbuvir and velpatasvir.

The main aim of the study was to investigate phytoconstituents and anti-helminthic activity in the canthium parviflorum root. Roots were collected and shade dried. Extraction was carried out by continues hot percolation method using ethylacetate and methanol as solvents. Both the extracts were subjected to phytochemical screening, using standard methods, where as anti-helminthic activity was evaluated at three different concentrations on Pheretima Postuma; the parameters like time of paralysis and time of death were determined. The extract showed significant activity in the dose dependent manner and methanolic root extract showed significant activity than that of standard drug Albendazole. This might due to the presence of secondary metabolite tannins which are responsible for the activity.

Release of drugs via the skin has been always a challenging part for research due to obstacle properties shown by the outermost layer of skin stratum corneum. In the previous two decades, the transdermal drug delivery system has turn into a established technology that offers important clinical repayment over additional dosage forms. Because transdermal drug delivery offers controlled as well as programmed rate of release of the drug into the patient, it is capable to maintain steady state blood concentration. It’s a advantageous form of drug delivery because of the apparent advantages e.g. suitable and pain-free self-administration for patients, prevention of hepatic first-pass metabolism and the GI tract for poorly bioavailable drugs than other routes of deliverance.Thepresent article reviews the choice of drug candidates and polymers, advantages, disadvantages of formulation, design and the methods of evaluation, augmentation techniques based on drug/vehicle optimization such as selection of drug, prodrugs and ion pairs, supersaturated drug solutions, vesicles, liposomes, particles and complexations. This review also emphases on the recent innovations in TDDS, which be able to used for the research and improvement of pharmaceutical dosage form intended for transdermal drug delivery.

Rubiacordifolia, belongs to family Rubiaceae, commonly known as Indian Madder and Manjistha in Sanskrit. It is perennial, herbaceous prickly climber with long and cylindrical root with a thin red bark. Various parts of Rubiacordifoliahave been suggested in the Indian system of medicine for a number of diseases. The roots and stems are well known source of Anthraquinones. The roots have also been reported to possess antioxidant, anti inflammatory, anticancer, immunomodulatory and hepatoprotective activities. This plant has been pharmacologically screened for anti-acne, anti-convulsant, anti-ulcer, anti-diabetic, anti-microbial, anti arthritic, anti-plateletand wound healing activities. This review will be helpful in the development of polyherbal formulation as well as for conducting further research to explore the therapeutic potential of Rubiacordifolia.

The aim of the present study is to minimize the local gastrointestinal irritation which is one of the major side effects of Piroxicam (PR) by the formulation  oral  stomach specific in situ gelling emulsion ingestion by kinetic control of drug release. Material and method: In situ emulgel were prepared by using castor oil as oil phase ,tween 80 and span 80 as emulsifiers, sodium alginate was used as gelling agent,  xanthan gum was used as release retardant ,calcium carbonate was used as cross linking agent, pH triggered ionic gelation is the mechanism involved in the present study. Various evaluation tests were done for all formulations Results: Formulation F9 containing 2.5% of sodium alginate, 2 % of CaCO3, 1 % of sodium bicarbonate and 0.8% of Xanthan gum was selected as optimized batch based on  Q10 86.02±0.17 %, floating time 122.15±2.47 sec and drug content 91.86±1.02 %. The release pattern of drug was found to follow Korsemeyer and Highuchi model. The DSC study exposed that there was no incompatibility. Pharmacodynamic study on Wistar rats were showed significant anti inflammatory and anti arthritic activity of the optimized formulation. Further, in vivo toxicity studies carried out in wistar rats revealed no signs of gastric ulceration upon prolonged dosing. Conclusion: It was concluded that the oral stomach specific In situ gelling emulsion of piroxicam could be an effective dosage form which minimize the gastric irritation by coating drug with castor oil and remains buoyant and control the drug release for 24hrs.

Efficient Helicobacter pylori elimination requires delivery of the antibiotic locally in the stomach. High dose of metronidazole (250 to 750 mg) is difficult to incorporate in floating tablets but can simply be given in liquid dosage form.  By keeping the above observation in mind, we made an attempt to build up a new raft forming oral in situ gelling system of metronidazole with improved residence time using sodium alginate as gelling polymer to eliminate H. pylori.  Methods: oral in situ gelling formulations were prepared using sodium alginate, xanthan gum, calcium carbonate, and sodium bicarbonate. Prepared formulations were evaluated for density, viscosity, floating lag time, floating duration, swelling index and in vitro drug release. Results. All formulations (F1–F12) showed floating within 180 s and had floating duration  of more than 24 h. every formulations showed excellent pourability. It was observed that concentration of sodium alginate and xanthan gum had major influence on floating lag time, cumulative percentage drug release and other evaluation parameters. The batch F11 was optimized since it have good pourability with extended release of 10 hrs. Conclusion: oral in situ gelling system of metronidazole  can be formulated by  use of sodium alginate as a gelling polymer and xanthan gum as release retardant to control  the drug release for more than  10 hrs.

The purpose of present research was to formulate and develop the patient friendly pantoprazole sodium fast dissolving tablets using sublimation method to achieve rapid dissolution. In this study, an attempt was made to fasten the drug release from the oral tablets by incorporating the superdisintegrants and camphor/ammonium bicarbonate as subliming agents. The prepared fast dissolving tablets were subjected to pre-compression analysis and evaluated for hardness, weight variation, friability, wetting time, water absorption ratio and disintegration time. From the results of in vitro drug release studies, the formulation F9 exhibited fast release profile of about 95.21% in 14 min and disintegration time 90 sec when compared with other formulations. For the optimized formulation F9, the initial dissolution rate was 38.82% / 2 min. Fourier transform infrared spectroscopy studies revealed that there was no possibility of interactions between drug and excipients. The present study demonstrated potential for rapid absorption, improved bioavailability, effective therapy and patient compliance.

Four diverse genotypes of Indian mustard (Brassica juncea L. Czern & Coss.)  and their crosses with Ogura restorer as the background were evaluated for eleven quantitative traits during winter (Rabi) 2011–12. The mean, range, phenotypic, genotypic and environmental variance, genotypic and phenotypic coefficient of variation, heritability in broad sense and genetic advance were calculated to differentiate the contribution made by each parameter in the final seed yield. The maximum value of phenotypic coefficient of variation was observed for no. of pods on main branch (36.23) followed by oil content (31.65), siliqua density on main shoot (30.72) and the highest genotypic coefficient of variation was observed for number of pods on main branch (33.49) followed by seed yield per plant (29.18) followed by oil content (28.89). Path coefficient analysis revealed that no. of pods on main branch (5.94) had the highest direct contribution towards seed yield per plant followed by 1000-seed weight (4.14) and siliqua density on main shoot (1.82) which suggested that selection for these traits would be quite effective in improving seed yield in Indian mustard. During the course of investigations, it was observed that the mean performance of F1’s (OR x RH 406 and OR x RH 832) was better than parental genotypes for no. of primary and secondary branches, no. of seeds per siliqua and seed yield per plant.

In the present study novel series of 3-((3-((6-benzoyl-1H-benz[d]imidazol-2-yl) amino)-5-mercapto-4H-1, 2, 4-triazol-4-yl)imino)substituted indolin-2-ones have been synthesized in good yields and characterized by IR, NMR and Mass spectral analyses. Compounds were evaluated for their preliminary in vitro cytotoxic activity against HCT-116 (colon), MCF-7 (breast), HeLa (cervical) and HepG2 (hepatocellular) cancer cell lines by standard MTT assay method, antioxidant activity by standard DPPH assay method and also were screened for  in vitro anti-inflammatory activity.

In the present study N-(2-oxosubstitutedindolin-3-ylidine)-2-phenylacetohydrazides (IV a-h) have been synthesized in good yields and characterized by IR, PMR and mass spectral analyses. Compounds were evaluated for their preliminary in vitro cytotoxic activity against HeLa cancer cell lines by standard MTT assay and also were screened for antioxidant activity by DPPH method. Our results shown that two of the analogues IVf and IVc  are potent antioxidant agents and IVb and IVg are potent cytotoxic agents.