Antioxidant.

In the present study novel series of 3-((3-((6-benzoyl-1H-benz[d]imidazol-2-yl) amino)-5-mercapto-4H-1, 2, 4-triazol-4-yl)imino)substituted indolin-2-ones have been synthesized in good yields and characterized by IR, NMR and Mass spectral analyses. Compounds were evaluated for their preliminary in vitro cytotoxic activity against HCT-116 (colon), MCF-7 (breast), HeLa (cervical) and HepG2 (hepatocellular) cancer cell lines by standard MTT assay method, antioxidant activity by standard DPPH assay method and also were screened for  in vitro anti-inflammatory activity.

To appraise the nephroprotective and antioxidant activity of hydroalcoholicCissampelospareira (C.pareira) whole plant extract using cisplatin induced nephrotoxic rats. C.pareirais a well know medicinal plant of Menispermaceae family which is widely used for ayurvedic medicine to treat various kidney complications. In the present study C.pareirawas observedfor its acute toxicity studies by using OECD guidelines no.425. The nephroprotective activity was evaluated by using cisplatin induced nephrotoxic rats. In-vivo antioxidant activity was evaluated by using glutathione and lipid peroxidation estimation in cisplatin induced rats. One way ANOVA followed by Dunnett’s test using Graph pad prism software. Statistical significance was set at P ≤ 0.05. From the acute toxicity study two doses were selected as 200 and 400 mg/kg p.o. body weight for the present study. C.pareira extract significantly increases the body weights, decrease the elevated urinary glucose levels in the urine, decrease the urea and creatinine levels in blood and increase the urinary creatinine levels in cisplatin induced nephrotoxic rats. In the in-vivo antioxidant study there was a dose dependent decreasing and increasing of lipid peroxidation, glutathione levels in hydroalcoholic extract treated groups respectively. The histopathological investigation was also supported nephroprotective activity of C.pareira. All the results were shown that the plants C.pareirahydroalcoholic extract has significant nephroprotective activity.

ABSTRACT: Hydroethanolic extract of Ricinus communis flowers was studied for treating diabetes induced oxidative stress in adult male swiss albino mice. The experimental animals were divided into four groups of seven mice each viz., Normal Control (NC), Diabetic Control (DC), Diabetic + R. communis Flower extract treated (FT) and Diabetic + Glibenclamide treated (GT). The groups DC, FT and GT were given a single intraperitoneal injection of Alloxan monohydrate (150 mg/kg body weight). Alloxan administration induced destruction of beta cells, hampering production of insulin and aggravating blood glucose levels. It also resulted in over-production of Reactive Oxygen Species (ROS) and thus oxidative stress. Groups NC and DC received normal saline while FT was given Ricinus Communis Flower Extract (RCFE) at 300 mg/kg body weight and GT was treated with Glibenclamide, for a period of 45 days. Fasting Blood Glucose (FBG) levels were observed at regular intervals. After the treatment period, the liver, kidneys and pancreas of the experimental animals were estimated for their antioxidative status. The antioxidant markers such as Catalase (CAT), Glutathione peroxidase (GPx), reduced Glutathione (GSH) and Superoxide dismutase (SOD) saw a variation from their normal values. Treatment of Alloxan induced diabetic mice with RCFE for 45 days lead to a significant (P