HPTLC

Quantitative estimation of temozolamide and its pharmaceutical dosage form by UV spectroscopy, RP-HPLC, HPTLC methods was developed. In the UV method (geometric method), temozolamide was quantified at 309nm, 325nm, 340nm in serum and water. The corrected absorbance was calculated. The Recovery studies was found to be 95.5-96.9%. In RP-HPLC method, the drug was resolved using a mobile phase methanol: buffer (5.0ml glacial acetic acid in 1000ml water) (70:30%v/v) on C18 column in isocratic mode. The retention time of temozolamide was found to be 7.30 min. Recovery studies was found to be 99.55-100.98%.  In HPTLC method, the chromatograms were developed by using a mobile phase Chloroform: glacial acetic acid: methanol (2:3:5% v/v) on precoated plate of silica gel 60F254 and quantified by densiometric absorbance mode at 254nm. The Rf value of Temozolamide was 0.47. Recovery studies of 98.99-100.6%, percentage relative standard deviation (%RSD less than 2%) and correlation coefficient (linearity range) that developed methods were accurate and precise. These methods can be employed for the routine analysis of capsules containing temozolamide. Key words: Temozolamide, RP-HPLC, HPTLC, UV spectrophotometry, validation.

Aloe gel is commonly used in herbal medicines. Many of the Aloe products available commercially, do not demonstrate beneficial effects, indicating the poor quality of the Aloe gel. Therefore, an efficient, reliable and accurate method is needed to evaluate the quality of Aloe products. The present research work discussed the development of a colorimetric and HPTLC method for the determination of Glucomannan (a marker compound for Aloe gel) in Aloe gels and its preparations. Shimadzu 1700 UV-Visible spectrophotometer was used for the colorimetric estimation. In the colorimetric method developed obeyed the Beer’s law in the concentration range of 50-90 μg-ml-1 with r2 of 0.999. CAMAG HPTLC instrument was used. The mobile phase comprised of n-butanol : ethanol : water : acetic acid (2 : 4 : 4 : 0.05 v/v) was used for the development of chromatogram. The densitometric scanning was done by TLC scanner III (CAMAG) in absorbance mode at the wavelength of 488 nm. After spraying with anisaldehyde sulfuric acid the system was found to give compact spots for glucomannan (Rf value of 0.69±0.02). The data obtained showed good linearity (r2 = 0.995) with respect to peak area in the concentration range of 400-1400 ng spot-1.

Iloperidone is a novel antipsychotic drug widely used to treat schizophrenia. Objective of this investigation was to develop a validated stability indicating high performance thin layer chromatographic method for the quantification of iloperidone in bulk and pharmaceutical dosage form. Aluminium backed TLC plates precoated with silica gel 60F-254 was employed as the stationary phase and n-propanol: chloroform (5:5 v/v) as the mobile phase. Densitometric analysis was performed at 275 nm in the reflectance mode. Compact spots of iloperidone with Rf value 0.36 were observed. Validation of the method as per ICH guidelines produced satisfactory results of linearity (r2>0.999), limit of detection (5.349 ng/spot), limit of quantification (16.2099 ng/spot), precision (< 2%), and accuracy (99.66 ±0.341 to 100.34±0.292%). Degradation products were found to be well separated from the pure drug with significantly different Rf values suggesting a stability indicating analysis method for the estimation of iloperidone in pharmaceutical preparations and as bulk drug. The proposed method is selective, sensitive, precise, and accurate. It is also simple, economic and time saving as compared to reported HPLC methods. 

Triphala guggulu contain the amala,baheda,and harde ,pipali, and guggulu in powder form which contain many phytoconstituents other than phenols(gallic acid,ellagic acid) and alkaloids(piperine) and Z-guggulusterone. phenolics are present in good amounts, and several as important anti-oxidant. Piperine is a bioavailability enhancer and guggulusterone as anticholesterimic. Looking into the importance of these ingredients, attempt has been made for simultaneous estimation of piperine, gallic acid, ellagic acid by using HPTLC methods  A simple HPTLC method has been developed for the estimation of galic acid, ellegic acid,piperine &z-gugggulu sterone from methanolic extract of marketed & lab preparation of triphala guggulu these compound in the extracts has been estimated by using HPTLC method the separation was performed on TLC Aluminum Plates precoated with silica gel 60F254,good separation was achieved in the mobile phase of toluene: ethyl acetate :formic acid: methanol(3:3:0.8:0.5)and densiometric determination of galic acid, ellegic acid carried out at 280 nm and piperine was carried out at 337nm&Z-guggulusterone was carried out at 248 nm & compare the these phytoconstituents with other marketed product

In the present investigations, methanolic extracts of four marketed Aegle marmelos formulations (F1, F2, F3, and F4) were prepared and subjected to simultaneous quantitative determination of two biologically active compounds; umbelliferone and quercetin. Analysis of umbelliferone and quercetin was performed on TLC aluminum plates pre-coated with silica gel 60F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of toluene: ethyl acetate: formic acid (6:4:1, v/v/v), and densitometric determination of these compounds was carried out at 300nm in reflectance/absorbance mode. The system was found to give compact spots for umbelliferone and quercetin with Rf value of 0.66 and 0.68, respectively. The present method was validated for precision, recovery, repeatability, and accuracy in accordance with International Conference on Harmonisation (ICH Q2) guidelines. Statistical analysis of the data showed that the method is reproducible and selective for estimation of umbelliferone and quercetin. This method may be used for routine quality control and standardization of the herbal drugs and there formulations. Key words: Aegle marmelos, Quercetin, Umbelliferone, HPTLC  

  An HPTLC method for simultaneous determination of Camylofin Dihydrochloride and Diclofenac Potassium in a pharmaceutical formulation has been developed and validated. The analyte were separated on silica gel 60F254 HPTLC plates with Benzene: Methanol: Ammonia in the ratio of 8.0:2.0:0.2, as mobile phase, after chamber saturation for 15 min. The development distance was 9 cm. The plate was then dried in air and scanned and quantified at the wavelength at 220 nm. The limits of detection were 25 μg mL–1 and 30 μg mL–1 for Camylofin Dihydrochloride and Diclofenac Potassium respectively. The limits of quantification were 50 μg mL–1 and 60 μg mL–1 for Camylofin Dihydrochloride and Diclofenac Potassium respectively. The method enables accurate, precise, and rapid simultaneous analysis of Camylofin Dihydrochloride and Diclofenac Potassium. It can be conveniently adopted for routine quality control analysis. Key words: HPTLC, Camylofin Dihydrochloride, Diclofenac Potassium.

  In the present communication, finger print of two ethano-botanically important Anisomeles species has been developed. A sensitive and reliable densitometric High Performance Thin Layer Chromatography (HPTLC) method has been developed for the quantification of quercetin, β-sitosterol, stigmasterol, catechin and ovatodiolide present in Anisomeles indica and Anisomeles malabarica.  Chromatographic analysis was performed using methanol, chloroform, acetone and ethanol extract of these plants were developed in the different solvents such as toluene, chloroform, ethyl acetate, methanol at various proportions. Detection and quantification of all phytoconstituents was done by densitometric scanning at different wavelengths. These finger prints would be helpful in the standardization of these species.   Key words: HPTLC, Anisomeles indica, Anisomeles malabarica, phytoconstituents, standardization

  Quantitative estimation of Trapidil and its pharmaceutical dosage form by HPLC, HPTLC and UV spectroscopy methods was developed. In the RP-HPLC method, the drug was resolved using a mobile phase phosphate buffer: acetonitrile (30:70%v/v) with pH adjusted to 3.5 using phosphoric acid on C18 column in isocratic mode. The retention time of trapidil was found to be 3.195 min.  In HPTLC method, the chromatograms were developed by using a mobile phase Methylene chloride: Methanol:  ammonia (8.5:1:0.5 v/v) on precoated plate of silica gel 60F254 and quantified by densiometric absorbance mode at 312nm. The Rf value of Trapidil was 0.28. In the UV method, trapidil was quantified at 221nm in acetronitrile. Recovery studies of 98.8-101.14%, percentage relative standard deviation (%RSD less than 2%) and correlation coefficient (linearity range) that developed methods were accurate and precise. These methods can be employed for the routine analysis of tablets containing trapidil.   Key words: Trapidil, RP-PHLC, HPTLC, UV spectrophotometry, validation

  A simple and sensitive high performance thin layer chromatography (HPTLC) method has been developed for the quantitative estimation of Tadalafil in its single component tablet formulation (20 mg). Tadalafil was chromatographed on silica gel 60 F254 TLC plate using chloroform: methanol (9:1, v/v) as mobile phase. Tadalafil showed Rf value 0.78 + 0.008 and scanned at 285 nm using a camag TLC scanner 3. The method was validated in terms of linearity (100 – 800 ng/spot), precision (intra-day variation, 0.38 to 0.81% and inter-day variation, 0.45 to 1.90%), accuracy (100.3 ± 0.76) and specificity. The limit of detection and limit of quantification for Tadalafil were found to be 28.11 ng/spot and 93.45 ng/spot, respectively. The developed method was successfully used for the assay of Tadalafil tablet formulation. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of Tadalafil in tablet dosage form.