Gallic acid

A simple and reliable high performance thin layer chromatographic (HPTLC) method has been developed and validated for the study of fingerprint chromatograms of the hexane , chloroform, ethyl acetate and aqueous extracts of leaves of Hemigraphis colorata (blume) H.G. Hallier .HPTLC separation of the all extracts was performed and scanned  at ultraviolet absorbance at 254 nm. A mobile phase composed of toluene: ethyl acetate: formic acid :methanol (3:6:1.6:0.4).The chromatogram obtained and the peak profile of the components collected by scanning could  made up the fingerprint of the  various  extracts from leaves of  Hemigraphis colorata and compared with antioxidants markers of flavonoids and phenolic acids. The fingerprint chromatograms had a good stability, precision, and reproducibility. The method is suitable for differentiation of extracts from the leaves of Hemigraphis colorata, and can be used as a quality control method for this plant.

A new series of novel substituted Gallic acid derivatives were synthesized by reacting methylated Gallic acid with hydrazine hydrate and then with various substituted aromatic aldehyde in mild and facile synthetic route to form various Schiff base derivatives.  Melting points of the synthesized compounds were determined by open capillary and are uncorrected. The purity of the compounds was checked using precoated TLC plates (MERCK, 60F) using chloroform: methanol (8:2) solvent system. The developed chromatographic plates were visualized under UV at 254nm. IR spectra were recorded using KBr on Josco FTIR model 8400 spectrophotometer, 1H NMR spectra in DMSO on a BRUKER FT-NMR instrument using TMS as internal standard. FAB mass spectra were recorded on JEOL SX 102 (DA-6000 mass Spectrometer) Data system using Argon (6KV.10MA) as the FAB gas. The designed compounds were screened for Antibacterial activity against Staphylococcus aureus and Escherichia coli and Antifungal activity against Aspergillus Niger in comparison with Ofloxacin and Fluconazole as standard to reveal the potency of synthesized derivatives. In accordance with the data obtained from antimicrobial activity, all the synthesized Schiff bases of substituted gallic acid have shown good activity against the tested microbes. Among these Schiff bases, compound bearing p-chloro group, o-chloro group, p-nitro group and m-nitro group has shown good activity against all the tested bacteria and fungi.

Triphala guggulu contain the amala,baheda,and harde ,pipali, and guggulu in powder form which contain many phytoconstituents other than phenols(gallic acid,ellagic acid) and alkaloids(piperine) and Z-guggulusterone. phenolics are present in good amounts, and several as important anti-oxidant. Piperine is a bioavailability enhancer and guggulusterone as anticholesterimic. Looking into the importance of these ingredients, attempt has been made for simultaneous estimation of piperine, gallic acid, ellagic acid by using HPTLC methods  A simple HPTLC method has been developed for the estimation of galic acid, ellegic acid,piperine &z-gugggulu sterone from methanolic extract of marketed & lab preparation of triphala guggulu these compound in the extracts has been estimated by using HPTLC method the separation was performed on TLC Aluminum Plates precoated with silica gel 60F254,good separation was achieved in the mobile phase of toluene: ethyl acetate :formic acid: methanol(3:3:0.8:0.5)and densiometric determination of galic acid, ellegic acid carried out at 280 nm and piperine was carried out at 337nm&Z-guggulusterone was carried out at 248 nm & compare the these phytoconstituents with other marketed product