rutin

A simple and reliable high performance thin layer chromatographic (HPTLC) method has been developed and validated for the study of fingerprint chromatograms of the hexane , chloroform, ethyl acetate and aqueous extracts of leaves of Hemigraphis colorata (blume) H.G. Hallier .HPTLC separation of the all extracts was performed and scanned  at ultraviolet absorbance at 254 nm. A mobile phase composed of toluene: ethyl acetate: formic acid :methanol (3:6:1.6:0.4).The chromatogram obtained and the peak profile of the components collected by scanning could  made up the fingerprint of the  various  extracts from leaves of  Hemigraphis colorata and compared with antioxidants markers of flavonoids and phenolic acids. The fingerprint chromatograms had a good stability, precision, and reproducibility. The method is suitable for differentiation of extracts from the leaves of Hemigraphis colorata, and can be used as a quality control method for this plant.

Euphorbia Hirta belonging to the Euphorbiaceae family contains more amounts of phenolic compounds and flavonoids which are responsible for the main pharmacological actions like anti-oxidant, anti-inflammatory, anti-dengue and anti-cancer. Considering the current importance of these ingredients, an attempt has been made for the simultaneous estimation of gallic acid, rutin and quercetin from euphorbia hirta by successive extraction involves the use of solvents in an order of increasing polarity in soxhlet extractor at 30-45 °C using 800 ml solvents for 5 hours in increasing polarity to isolate the active constituents without other interferences. Hence we proposed to develop easy, rapid, accurate, precise and reliable analytical HPTLC method for the standardization of Euphorbia hirta(L) using gallic acid, rutin and quercetin as phytochemical markers from its methanolic extract and herbal capsule formulation. The separation was performed on TLC aluminum Plates precoated with silica gel 60F254, good separation was achieved in the mobile phase of butyl acetate: 1,4-dioxane (5:5% v/v) and densitometric determination of gallic acid, rutin and quercetin was carried out at 266nm.The linear regression data showed a good linearity in the concentration range of 136-748ng/spot of gallic acid, rutin and quercetin with a good correlation coefficient of 0.9989. Limit of detection was found to be 102 ng/spot of gallic acid,17ng/spot of rutin and 68ng/spot of quercetin. The limit of quantification for the estimation of gallic acid, rutin and quercetin was found to be 136ng/spot.