Validated Estimation of Temozolamide In Pure, Biological Sample By UV-Spectroscopic, RP-HPLC And HPTLC Methods

Quantitative estimation of temozolamide and its pharmaceutical dosage form by UV spectroscopy, RP-HPLC, HPTLC methods was developed. In the UV method (geometric method), temozolamide was quantified at 309nm, 325nm, 340nm in serum and water. The corrected absorbance was calculated. The Recovery studies was found to be 95.5-96.9%. In RP-HPLC method, the drug was resolved using a mobile phase methanol: buffer (5.0ml glacial acetic acid in 1000ml water) (70:30%v/v) on C18 column in isocratic mode. The retention time of temozolamide was found to be 7.30 min. Recovery studies was found to be 99.55-100.98%.  In HPTLC method, the chromatograms were developed by using a mobile phase Chloroform: glacial acetic acid: methanol (2:3:5% v/v) on precoated plate of silica gel 60F254 and quantified by densiometric absorbance mode at 254nm. The Rf value of Temozolamide was 0.47. Recovery studies of 98.99-100.6%, percentage relative standard deviation (%RSD less than 2%) and correlation coefficient (linearity range) that developed methods were accurate and precise. These methods can be employed for the routine analysis of capsules containing temozolamide. Key words: Temozolamide, RP-HPLC, HPTLC, UV spectrophotometry, validation.