tablet

In the current investigation we formulated and evaluates matrix tablet using modified gum of Pistacia lantiscus gum (PLMG) as natural matrix forming agent used in various successively increasing concentration. The pre-compression study of the powder blends of drug, PLMG and other excipients were done by calculating bulk density, tapped density, angle of repose and carr’s index (% compressibility) and hausner’s ratio. The tablets using PLMG as matrix forming agent were prepared by direct compression method and prepared tablets were evaluated for thickness, hardness, weight uniformity, friability and content uniformity and were found according to the official guidelines by pharmacopoeia. The swelling behavior of prepared matrix tablets was studied using for 12 hours at 37 ±0.2 0C, it was fond that the drug to modified gum ratio of 1:2 was found to be optimum swelling with the sustained release of model drug up to 97.12 in matrix tablet formulation. The results revealed, that modified fraction of Pistacia lantiscus gum can be used as a drug release modifier to delay as the rate of drug release of which depended on the amount of gum composition, as the concentration of gum was increased there was sustain the drug release.

Bilayer tablets are planned by way of one layer of drug for immediate release with the second layer designed to release drug later, either as a second dose or in an sustained release form. The bilayer tablets with two incompatible drugs can also be prepared by compressing separate layers of each drug so as to minimize area of contact between two layers of two API. To manufacture sufficient tablet formulation, definite necessities such as sufficient mechanical strength and desired drug release profile must be meet. At times, this may be difficult task for formulator to achieve these conditions especially in bilayer tablet formulation where double compression technique is involved, because of poor flow and compatibility characteristic of the drug which will result in capping and/or lamination. The compaction of a material involves both the compressibility and consolidation.

A simple and sensitive high performance thin layer chromatography (HPTLC) method has been developed for the quantitative estimation of Donepezil HCL in its bulk and tablet dose tablet formulation (5 mg). Donepezil HCL was chromatographed on silica gel 60 F254 TLC plate using  methanol : toluene (2:8, v/v) as mobile phase. Donepezil HCL showed Rf value 0.54 + 0.008 and scanned at 245 nm using a camag TLC scanner 3. The method was validated in terms of linearity (100 – 800 ng/spot), precision (system precision = 0.0123 and method precision = 0.0084), accuracy (100.3 ± 0.76) and specificity. The limit of detection and limit of quantification for Donepezil HCL were found to be 1.72 ng/spot and 2.07 ng/spot, respectively. The developed method was successfully used for the assay of Donepezil HCL tablet formulation. This method also contain forced degradation studies for standard and tablet. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of Donepezil HCL in tablet dosage form.

Accurate, precise, rapid and economical first order derivative spectroscopic method was developed and validated for the estimation of Cefixime and levofloxacin in tablet. The wavelengths selected for quantitation were 289.45 nm for levofloxacin (zero cross for cefixime) and 317.0 nm for cefixime (zero cross for levofloxacin). Linearity for detector response was observed in the concentration range of 2-12 μg/ml for both Cefixime and Levofloxacin using methanol as a solvent with correlation coefficient 0.991 and 0.993 respectively. The proposed method was successfully applied for the simultaneous estimation of both drugs in tablet.

The present manuscript describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of nebivolol and hydrochlorothiazide in combined tablet dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra were obtained in methanol and the determinations were made at 270.5 nm (ZCP of hydrochlorothiazide) for nebivolol and 282.5 nm (ZCP of nebivolol) for hydrochlorothiazide. The linearity was obtained in the concentration range of 5-100 μg/ml for nebivolol and 2-14 μg/ml for hydrochlorothiazide. The mean recovery was 100.04 + 0.93 and 99.87 + 1.16 for nebivolol and hydrochlorothiazide, respectively. The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of nebivolol and hydrochlorothiazide in pharmaceutical tablet dosage form. The results of analysis have been validated statistically and by recovery studies. Key words: Nebivolol, hydrochlorothiazide, recovery, first order derivative spectrophotometric method, tablet, validation.

  The present manuscript describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of nebivolol and hydrochlorothiazide in combined tablet dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra were obtained in methanol and the determinations were made at 270.5 nm (ZCP of hydrochlorothiazide) for nebivolol and 282.5 nm (ZCP of nebivolol) for hydrochlorothiazide. The linearity was obtained in the concentration range of 5-100 μg/ml for nebivolol and 2-14 μg/ml for hydrochlorothiazide. The mean recovery was 100.04 + 0.93 and 99.87 + 1.16 for nebivolol and hydrochlorothiazide, respectively. The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of nebivolol and hydrochlorothiazide in pharmaceutical tablet dosage form. The results of analysis have been validated statistically and by recovery studies.     Key words: Nebivolol, hydrochlorothiazide, recovery, first order derivative spectrophotometric method, tablet, validation.

  A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of diazepam and propranolol hydrochloride in pharmaceutical tablet formulation. The separation was achieved on Phenomenex C18 column (250 mm i.d., 4.6 mm, 5 µm particle size) using methanol: acetonitrile: water (50 : 25 : 25, v/v/v, pH adjusted to 2.8 ± 0.05 with ortho- phosphoric acid) as the mobile phase at a flow rate of 1.0 ml min-1. The quantification was achieved with PDA detector at 235 nm. The injection volume was 20 µl. The retention times of diazepam and propranolol hydrochloride were 5.38 ± 0.29 min and 3.80 ± 0.15 min, respectively. The method was validated for linearity, precision, specificity, robustness and recovery according to the ICH guidelines. The linearity was obtained in the concentration range of 0.1-5.0 µg/ml for both drugs with mean recovery of 100.3 ± 0.47 and 100.2 ± 0.78 % for diazepam and propranolol hydrochloride, respectively. The limit of detection and quantification for diazepam were 0.015 and 0.050 µg/ml, respectively and for propranolol hydrochloride were 0.014 and 0.045 µg/ml, respectively. The method was found to be simple and highly sensitive and can be useful in the routine quality control of diazepam and propranolol hydrochloride in bulk manufacturing and pharmaceutical dosage forms.   Key words: Diazepam, propranolol hydrochloride, RP-HPLC, validation, simultaneous, tablet

  The present manuscript describe simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of Ofloxacin and Cefpodoxime proxetil in combined tablet dosage form. The method is based on the simultaneous equations for analysis of both the drugs using methanol as solvent. Ofloxacin has absorbance maxima at 297 nm and cefpodoxime proxetil has absorbance maxima at 236.2 nm in methanol. The linearity was obtained in the concentration range of 2-12 μg/ml and 4-24 μg/ml for Ofloxacin and Cefpodoxime proxetil, respectively. The concentrations of the drugs were determined by using simultaneous equations at both the wavelengths. The mean recovery was 99.63 ± 0.47 and 99.57 ± 0.36 for Ofloxacin and Cefpodoxime proxetil, respectively. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The suitability of this method for the quantitative determination of Ofloxacin and Cefpodoxime proxetil was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Ofloxacin and Cefpodoxime proxetil in pharmaceutical tablet dosage form. The results of analysis have been validated statistically and by recovery studies.   Key words: Cefpodoxime proxetil, Ofloxacin, recovery, simultaneous equations method, tablet, validation.

  A simple and sensitive high performance thin layer chromatography (HPTLC) method has been developed for the quantitative estimation of Tadalafil in its single component tablet formulation (20 mg). Tadalafil was chromatographed on silica gel 60 F254 TLC plate using chloroform: methanol (9:1, v/v) as mobile phase. Tadalafil showed Rf value 0.78 + 0.008 and scanned at 285 nm using a camag TLC scanner 3. The method was validated in terms of linearity (100 – 800 ng/spot), precision (intra-day variation, 0.38 to 0.81% and inter-day variation, 0.45 to 1.90%), accuracy (100.3 ± 0.76) and specificity. The limit of detection and limit of quantification for Tadalafil were found to be 28.11 ng/spot and 93.45 ng/spot, respectively. The developed method was successfully used for the assay of Tadalafil tablet formulation. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of Tadalafil in tablet dosage form.