Kiran

Steven Johnson syndrome is a severe and potentially lethal disease due to an immune-complex-mediated hypersensitivity reaction involving mucous membranes and skin. Steven Johnson syndrome and toxic epidermal necrolysis (TEN) are life-threatening diseases characterized by the detachment of skin and mucous membranes. It has been a common belief that herbal drugs do not cause such as much as side effects and toxicities as pure chemicals. Recently, SJS associated with herbal medicine has been reported. This paper aims to report a case of SJS marked with skin manifestations following the use of herbal drugs for the treatment of Alcohol withdrawal and Jaundice. A 28 years male old patient was admitted with complaints of Fever, Vomiting, and Rashes over the body. The patient had received herbal drugs for the treatment of Jaundice and Alcohol dependence. Herbal medicine still plays an important role in the Indian Healthcare system as it is one of the most ancient treatment systems in the Indian Healthcare system. Further studies may require to evaluate the characteristics of the general population and to identify any predisposing factors to SJS as recommended.

Acquired Immunodeficiency Syndrome (AIDS) is a clinical infirmity that is a result of infection with Human immunodeficiency virus (HIV), which causes intense immunosuppression. Many compounds of plant origin that inhibit HIV during various stages of cycle theses include several alkaloids, Carbohydrates, Xanthenes and tannins. These compounds have the capability to come up as a medication for therapy of HIV infection. Herbal medication provides rational means for the therapy of Aids, the Herbal drugs which are utilized in Nut, Turmeric, Asoka, Sarpagandha, Cotton Seed, Arjuna. So, the focus of this review article is to pinpoint possessing activity against HIV objective of providing an effective approach for prevention of transmission and therapy of these diseases.

The present research is focused on development of mouth dissolving tablet of Lornoxicam using novel superdisintegrants from natural resources. The research is carried out to potentiate the use of natural excipients instead of synthetic ones. Lornoxicam B cyclodextrin complex is formed as it increases the solubility of drug and to mask the taste of drug while having many advantages such as improve dissolution, and bioavailability. Tablets were prepared using natural superdisintegrants like gum karaya, Plantago ovata husk. and synthetic superdisintegrants like Crospovidone, Kyron T-314, Croscarmellose Sodium. Tablet containing 6 % of gum karaya shows better results over the formulation containing synthetic or other natural superdisintegrants like Plantago ovata husk. The formulated tablet melts in mouth within fraction of seconds with promising release of drug. The present study demonstrated potentials for rapid absorption, improved bioavailability, effective therapy, acceptable taste and patient compliance. The accelerated stability study of batch (F2) revealed that no significant change in physical properties and could be considered as stable formulation even after 3 months.

A simple, sensitive, selective and rugged liquid chromatography coupled with mass spectrometry (LCMS/MS) method for quantification of Diltiazem and its metabolites, N-desmethyl Diltiazem, desacetyl Diltiazem in human plasma was developed and validated. The chromatography was developed using Luna 5 μ, C18, 100×4.60 mm column having a mobile phase of Acetonitrile: 0.1% formic acid (85:15 % v/v). The flow rate was 0.5 ml/min at a column temperature of 50 ± 5º C. Electron spray ionization technique in positive mode was selected to improve the selectivity and sensitivity required for this application. The retention times of Diltiazem, desmethyl Diltiazem, desacetyl Diltiazem were 2.5, 2.0 and 2.5 minutes respectively. The method was validated for linearity, precision, accuracy, specificity, sensitivity, matrix effect, dilution integrity, ruggedness, injection reproducibility and stability. Calibration curves during the course of validation were found to be linear for Diltiazem, desmethyl Diltiazem, desacetyl Diltiazem in the ranges of 0.604-603.902, 0.303-303.274 and 0.299-299.489 ng/mL with correlation coefficient ≥ 0.9969, 0.9958 and 0.9970 respectively and by using a 1/x2 weighted least square regression analysis of standard plots associated with ten point calibration standards. The precision and mean accuracy were within the acceptable limits. Keywords: Diltiazem; desmethyl Diltiazem, desacetyl Diltiazem; LCMS/MS; Validation.

The purpose of this research and development was to develop the elevated susceptible, speedy, stable and reproducible extraction method with precise results. At the same time, this method would be efficient in analyzing the large numbers of plasma samples obtained for bioequivalence studies. The chromatography was performed by using column ZIC®-HILIC, 4.6×150 mm, 5µ. Nimodipine was used as internal standard. The solid phase extraction was used to extract Acamprosate and Nimodipine (Internal standard) from plasma samples. The calibration curve was relied on the concentration of 10.01 ng/mL to 709.36 ng/mL for Acamprosate in human plasma Keywords: Bioequivalence study, LCMS and solid phase extraction  

The aimed to formulate and evaluate herbal shampoo because synthetic may causes the adverse effect on hair and scalp. The herbal shampoo was formulated by extracting murraya koenigi, sapindusmukorossias foaming agent, also addition of preservative agent. Citric acid used as viscosity modifier and pH adjusting agent and glycerin used as conditioning agent. HPMC(hydroxyl propyl methyl cellulose) used as thickening agent. The formulation at laboratory scale was done and evaluated for number of parameters such as pH, foam formation, viscosity, conditioning and wet ability were evaluated, and also to ensure its safety and efficacy

Now days more importance has been given to use medicinal plant products in healthcare system.it fulfills worldwide need of alternative medicine so traditional systems of medicine becomes more and more popular. It involves Proper combination of modern scientific techniques and traditional knowledge.  The quality of herbal products is checked through  stability testing studies which depends on various  factors, such as temperature, humidity, light, oxygen, moisture, other ingredient , microbial contamination, trace metal contamination, leaching from the container, etc. Therefore such studies involves various types of evaluation such as chemical, physical, microbiological, therapeutic and toxicological studies can serve as an important tool in stability studies. Standardization of herbal drugs means confirmation of its identity, Quality and purity. The present study includes review of various standardization parameters with their effectivity towards the quality herbal drugs. Present article also overviews various well-designed methodologies, techniques such as Chromatography, Spectroscopic tech. for the standardization of herbal raw materials and herbal formulations.

This study aimed to develop hydrophilic matrix based controlled release  gastroretentive drug delivery system of Lamivudine floating matrix tablets, which after oral administration are designed to prolong the gastric residence time, increase the drug bioavailability and diminish the side effects of irritating drugs. FTIR studies revealed that there is no interaction between the drug and polymers used for the formulation. Among all the formulations F21 containing HPMC K100M, Carbopol 934P, Polyox WSR 303 and sodium bicarbonate, as gas generating agent was selected as optimized formulation based on physico chemical properties, floating lag time (34 sec) and total floating time (>24 h). From in vitro dissolution studies, the optimized formulation F21 showed drug release of 99.36±5.36%, whereas 92.36±5.02% of the drug was released from the marketed product within 24h. From in vivo bioavailability studies, after oral administration of floating tablet containing 100 mg Lamivudine, the Cmax, Tmax, and AUC0–∞ of optimized gastroretentive formulation were found to be 32.11±3.16 µg/mL, 8.00±1.26 h and 225±28.14 µg*h/ml, respectively. Cmax and AUC values of optimized formulation were found to be significantly higher than of marketed product, where longer gastric residence time is an important condition for prolonged or controlled drug release and also for improved bioavailability. Hence, gastro retention can be a promising approach to enhance bioavaiilability of Lamivudine with narrow absorption window in upper GIT.

Autophagy is an intracellular   has demonstrated that autophagy plays a wide variety of physiological and pathophysiological roles, which are sometimes complex. Autophagy consists of several sequential steps sequestration, transport to lysosomes, degradation, and utilization of degradation products and each step may exert different function. In this review, the process of autophagy is summarized, and the role of autophagy is discussed in various diseases like Cancer, Neurodegenerative disease etc. 

The novel Pyrazoline derivatives were prepared  by cyclisation of substituted chalcone derivatives in the presence of 2, 4 dintro phenyl hydrazine hydrate. Structural elucidation of all synthesized  derivatives were done by spectral analysis ( IR , NMR and Mass spectroscopy). All synthesized derivatives  screened for their anticancer activities by MTT assay and  these prepared derivatives exhibits promising anticancer activities.