anticancer activity

The novel Pyrazoline derivatives were prepared  by cyclisation of substituted chalcone derivatives in the presence of 2, 4 dintro phenyl hydrazine hydrate. Structural elucidation of all synthesized  derivatives were done by spectral analysis ( IR , NMR and Mass spectroscopy). All synthesized derivatives  screened for their anticancer activities by MTT assay and  these prepared derivatives exhibits promising anticancer activities.

Adhatoda vasica Nees (Acanthaceace) is a well known medicinal plant. Its diverse medicinal activities include cardiovascular protection, abortifacient, antitubercular, antimutagenic, antiulcer, antiasthmatic activities, hepatoprotective, antibacterial and anti cancer activities. It is commonly used in original and traditional folk medicine system. In the prior times, many people used plants to cure diseases just by experimental data without knowing anything about its compounds. A number of evidence has been documented to demonstrate the probability of these plants in pharma industry. In recent years Adhatoda vasica an important medicinal plant that has been extensively used in pharmacological analysis and toxicological studies was found and these phytochemicals were considered to be greater importance in pharma industry. The present study was carried out to evaluate the anticancer activity of the compound isolated from ethyl acetate fractions of Adhatoda vasica and it was found to very good anticancer action against liver cancer. The compound isolated from ethyl acetate fraction of Adhatoda vasica flowers was tested for its anti cancer activity against liver cancer HePG2 cell line by MTT assay. The CTC50 value of sample was 228.5µg/ml against liver cancer HePG2 cell lines. Significant results were observed thereby proving the use of this plant in the traditional system of medicine.

Chemistry of heterocyclic organic compounds is as logical as that of aliphatic/aromatic compounds and of great interests both from the theoretical as well as practical synthetic view to design novel compounds that becomes one of the most important areas of research today. Electron-flux of six member/annulated N heterocycles plays vital roles to exhibit diverse potent biological activities from pharmaceuticals to industrial materials. The Pyrimidine and fused pyrimidine annulated rings in the chemistry of biological systems has attracted much attention due to availability in the substructures of therapeutic imperative natural products. As a result of their prominent and remarkable pharmacological activity, pyrimidine derivative’s intensive research has been made especially focused on its anticancer activity. The present review gives brief information about anticancer activity of assorted six member/annulated pyrimidine derivatives.

Three novel Mannich bases 5-[(4-chlorophenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione :CBzBAPz(NM), 5-[(4-nitrophenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione: NBzBAPz(NM) and 5-[(phenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione: BzBAPz(NM) are prepared by the condensation of N-methyl piperazine with p-chlorobenzaldehyde / p-nitro benzaldehyde / benzaldehyde and barbituric acid and  characterized by physico-chemical,  spectroscopic, and powder X-ray diffraction studies. Further, Cu (II), Cd (II) and Pd (II) complexes of titled ligands are synthesized and characterized. Based on the experimental results, the chelating (bidentate) behavior of the ligands is accessed. The X-band e.p.r.spectrum, electrochemical studies of the Cu (II) complex have been carried out and discussed. Three novel Mannich bases and their complexes exhibit comparable potential cytotoxicity against breast cancer cell line (MCF-7), particularly, strong cytotoxicity is observed in the case of palladium. Morphological features of cells used for the study is photographed. Use of fluorochromesacridine orange and ethidium bromide reveals apoptotic morphological features.