MTT assay.

The novel Pyrazoline derivatives were prepared  by cyclisation of substituted chalcone derivatives in the presence of 2, 4 dintro phenyl hydrazine hydrate. Structural elucidation of all synthesized  derivatives were done by spectral analysis ( IR , NMR and Mass spectroscopy). All synthesized derivatives  screened for their anticancer activities by MTT assay and  these prepared derivatives exhibits promising anticancer activities.

Plants have been used as medicines for thousands of years. They have always been used as a rich source of biologically active drugs. According to WHO (World Health Organization) report, about 80% of the population, mostly in developing countries still depends on traditional medicinal system for their primary health care. The present investigation is focused on the phytochemical investigation of abutilon indicum species for anticancer activity. The Abutilon L. genus of the Malvaceae family comprises about 150 annual or perennial herbs, shrubs or even small trees widely distributed in the tropical and subtropical countries. The aqueous extract of the A. indicum L. showed significant cytotoxic activity against both the selected cancer cell lines viz. human breast cancer cell line MCF 7 and human lung cancer cell line A 549.

Three novel Mannich bases 5-[(4-chlorophenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione :CBzBAPz(NM), 5-[(4-nitrophenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione: NBzBAPz(NM) and 5-[(phenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione: BzBAPz(NM) are prepared by the condensation of N-methyl piperazine with p-chlorobenzaldehyde / p-nitro benzaldehyde / benzaldehyde and barbituric acid and  characterized by physico-chemical,  spectroscopic, and powder X-ray diffraction studies. Further, Cu (II), Cd (II) and Pd (II) complexes of titled ligands are synthesized and characterized. Based on the experimental results, the chelating (bidentate) behavior of the ligands is accessed. The X-band e.p.r.spectrum, electrochemical studies of the Cu (II) complex have been carried out and discussed. Three novel Mannich bases and their complexes exhibit comparable potential cytotoxicity against breast cancer cell line (MCF-7), particularly, strong cytotoxicity is observed in the case of palladium. Morphological features of cells used for the study is photographed. Use of fluorochromesacridine orange and ethidium bromide reveals apoptotic morphological features.