antimicrobial activity

Bakreshwar hot springs have geological significance, and are known to have curative properties for any diseases. In this study, antimicrobial activities of the hot spring Bakreshwar water were assessed against MDR and ATCC microorganisms. A range of bacterial strains, including Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Escherichia coli (MDR), Klebsiella sp. (MDR), Acinetobacter sp. (MDR) and Pseudomonas sp. (MDR) were used to evaluate the antimicrobial activity of the water. The results showed that the hot spring water expressed significant antimicrobial activity against all tested MDR and ATCC microorganisms. High temperature and strong alkaline pH (9.2) of the hot spring water, the presence of minerals and the physical and chemical agents in the water are probably responsible for the antimicrobial activities that were observed. The findings lead us to believe that the hot spring water from Bakreshwar might be a source of natural antimicrobial properties, which could be utilized for the development of new drugs for treating infections caused by resistant bacteria. Further studies are required to confirm the specific mineral, chemical or physical agent that are responsible for the observed activity.

Natural remedies are more acceptable in the belief that they are safer with fewer side effects than the synthetic ones. Herbal formulations have growing demand in the world market. The present work deals with the development & evaluation of the herbal anti-acne face wash containing aqueous extract of Luffa cylindrica seed oil, neem leaves (Azadirachta indica), Pumpkin. Although various topical herbal formulations for acne are available in the market, we propose to make pure herbal formulation without using any synthetic soap . The plants have been reported in literature having good anti-microbial, anti-oxidant & anti-inflammatory activity also and saponins present in the Luffa cylindrica seeds used as a base to form the soap. Prepared formulations were evaluated for various parameters like colour, appearance, consistency, washability, pH & spreadability. The formulation was compared with the marketed preparation.

Present report is the successful synthesis, spectral characterization and in-vitro antimicrobial evaluation of a series of azomethine and novel 2-azetidinone derivatives. Two cyanoethylated tertiaryaminobezaldehydes A and B were prepared by cyanoethylation of the corresponding aromatic primary amines followed by formylation in presence of POCl3 and DMF. These cyanoethylated tertiaryaminobenzaldehydes on condensation with different aromatic primary amines afforded azomethines SB1-SB14, which on cyclization with chloroacetylchloride and triethylamines in 1,4-dioxan gave new 2-azetidinone BL1-BL14. All compounds were prepared by reported methodology and characterized by elemental analysis, FT-IR and 1HNMR data. Further screened in-vitro for antimicrobial activity against S. aureus, B. subtilis, P. vulgaris, E. coli, A. niger and A. fumigatus. Most of the compounds showed significant activity against tested pathogens. The work shows the emergence of a new series of compounds in the field of antimicrobials.

This study was performed to evaluate the antimicrobial activity of Hibiscus vitifolius. The ethyl acetate fraction of Hibiscus vitifolius were shown to possess an antimicrobial activity against bacteria and fungi, viz. Six bacterial strains were S. typhi, E. coli, E. faecalis, B. cereus, B.Substilis, Lacto bacillus and two fungal strains C.lunata, C.albicans  by using disc diffusion method. The anti bacterial activity of ethyl acetate fraction is almost comparable with standard solvent control Chloramphenicol. The anti fungal activity of ethyl acetate fraction extract is almost comparable with standard solvent control Fluconazole. Further studies are highly needed for future drug development.

Fifty pus samples were collected from burn patients with age ranging 10-55 years with invasive wound infection using sterile cotton swabs. Pure culture were isolated, maintained, preserved and subjected for morphological characterization, accomplished by studying the colony characteristics on different culture plates followed by simple, negative and Gram staining. The cultures then also subjected for biochemical characterization performing various biochemical tests Recovered pathogens were mostly Gram negative. The most common causative agent was found to be Pseudomonas aeruginosa (39.5%), followed by Staphylococcus aureus (25.5%), Acinetobacter baumannii (10%), Klebsiella spp (4.2%), Proteus vulgaris (3.5%) and Escheritia coli (2%). All the previously synthesized compounds were tested on all the isolated pathogens using disc diffusion method and Ciprofloxacin was used as standard drug. The results revealed that out of five, three compounds (1-3) were found to possess significant antimicrobial potential against all pathogens and compounds 4, 5 were also exhibited good activity against E. coli.

In the present study a series of 3-(2-Hydroxy-3, 5-dichlorophenyl)-5-(4-chloro phenyl) isoxazole (8a) and 3-(2-Hydroxy-3, 5-dichlorophenyl)-5-(2, 4-chloro phenyl) isoxazole (8b) have been synthesized by refluxing the chalconedibromide (6a) and (6b) with hydroxyl amine hydrochloride in ethanol containing catalytic amount of piperidine. The formation of the above synthesized compounds was confirmed on the basis of their chemical tests and spectral analysis. The synthesized heterocycles then screen for their antimicrobial activity against some kahrip plant pathogens viz. AlternariaMacrospor, XanthomonasCampestris, Pseudomonas syringae and Fusarium Spp. From the above study the result revealed that isoxazole derivatives shows good to moderate antimicrobial activity against plantpathoges.

A series of novel schiff bases containing N-benzyl piperidine moiety (1a-d) were synthesized by the reaction of N-benzylpiperidine-4-carbaldehyde with suitable aromatic amines. And synthesized schiff bases were converted to a series of novel 4-thiazolidinones (2a-d) by the reaction of Schiff base with mercaptoacetic acid. The chemical structures of the synthesized compounds were confirmed by using spectroscopic and chemical analysis techniques. All the above compounds were screened for their antimicrobial activity against some selected pathogenic microorganism. Good level of antimicrobial activity has been displayed by both categories of compounds against tested pathogenic microorganism. Thiazolidinone derivatives (2a-d) showed higher activity than schiff base derivatives (1a-d).