Klebsiella sp.

Bakreshwar hot springs have geological significance, and are known to have curative properties for any diseases. In this study, antimicrobial activities of the hot spring Bakreshwar water were assessed against MDR and ATCC microorganisms. A range of bacterial strains, including Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Escherichia coli (MDR), Klebsiella sp. (MDR), Acinetobacter sp. (MDR) and Pseudomonas sp. (MDR) were used to evaluate the antimicrobial activity of the water. The results showed that the hot spring water expressed significant antimicrobial activity against all tested MDR and ATCC microorganisms. High temperature and strong alkaline pH (9.2) of the hot spring water, the presence of minerals and the physical and chemical agents in the water are probably responsible for the antimicrobial activities that were observed. The findings lead us to believe that the hot spring water from Bakreshwar might be a source of natural antimicrobial properties, which could be utilized for the development of new drugs for treating infections caused by resistant bacteria. Further studies are required to confirm the specific mineral, chemical or physical agent that are responsible for the observed activity.

Emerging Multidrug-resistant problem is a major concern. Klebsiella sp. can lead to a wide range of disease causing pathogen namely  pneumonia, urinary tract infections, septicemia, meningitis, diarrhea and soft tissue infections and development of MDR against commonly used antibiotics making the case difficult to manage. Total 525 Clinical samples were screened for isolation of Klebsiella sp. Total 36 isolates belonging to Klebsiella sp. Were obtained. 29(8.06%) isolates were associated with urinary tract infection (UTI), 6(12%) with sputum and 1(1.54%) was associated with blood. Antibiogram study of these isolates revealed that all these isolates are resistant to several antibiotics out of 34 antibiotics tested. Cold and hot acacia leaf petroleum ether extract, cold and hot acacia leaf chloroform extract, cold and hot acacia leaf acetone extract, cold and hot acacia leaf methanol extract and cold and hot acacia leaf water extract were used for synergistic study. Qualitative phytochemical analysis these leaf extracts showed the prominent presence of alkaloids, Carbohydrates and glycosides, proteins and amino acids and phytosteroids. The studies on these extracts of Acacia polycantha and antibiotics on susceptibility of resistant Klebsiella isolates showed that both cold and hot solvent extracts are effective on antibiotics. The effectiveness shown by these extracts may be useful in evaluating the efficacy of combination therapy against MDR-Klebsiella sp.