Multidrug resistance

Emerging Multidrug-resistant problem is a major concern. Klebsiella sp. can lead to a wide range of disease causing pathogen namely  pneumonia, urinary tract infections, septicemia, meningitis, diarrhea and soft tissue infections and development of MDR against commonly used antibiotics making the case difficult to manage. Total 525 Clinical samples were screened for isolation of Klebsiella sp. Total 36 isolates belonging to Klebsiella sp. Were obtained. 29(8.06%) isolates were associated with urinary tract infection (UTI), 6(12%) with sputum and 1(1.54%) was associated with blood. Antibiogram study of these isolates revealed that all these isolates are resistant to several antibiotics out of 34 antibiotics tested. Cold and hot acacia leaf petroleum ether extract, cold and hot acacia leaf chloroform extract, cold and hot acacia leaf acetone extract, cold and hot acacia leaf methanol extract and cold and hot acacia leaf water extract were used for synergistic study. Qualitative phytochemical analysis these leaf extracts showed the prominent presence of alkaloids, Carbohydrates and glycosides, proteins and amino acids and phytosteroids. The studies on these extracts of Acacia polycantha and antibiotics on susceptibility of resistant Klebsiella isolates showed that both cold and hot solvent extracts are effective on antibiotics. The effectiveness shown by these extracts may be useful in evaluating the efficacy of combination therapy against MDR-Klebsiella sp.

Antimicrobial’s efficiency is critically compromised by the emergence and spread of antimicrobial resistance, yet they are valuable therapeutics. Investigations were carried to test and analyze the antimicrobial susceptibility of E. coli isolates from Ajmer poultry farm. 24 tissue samples containing 6 liver, 12 small intestine, and 6 large intestine samples were processed for isolation and identification and characterization of pathogenic E. coli from 12 fowls of 4 selected poultry farms of Ajmer suspected for colibacillosis and subjected to detailed bacteriological and biochemical examination. 24 E. coli isolates were isolated following standard procedures. E. coli isolates found to be highly resistant to some of the classical drugs like tetracycline, amoxy clav, carbenicillin, cotrimoxazole and cefopodoxime and found to be highly sensitive (100%) to the remaining drugs, which is disclosed by antibiogram study. The most ineffective antibiotic drug was found to be tetracycline for all the E. coli isolates, as all these microbes were found 100% resistant to it. Multiple resistance to at least 2 to 4 antibiotics were observed in all the 24 isolates. 50% of the isolates were resistant to at least 4 antibiotics and 75% to at least 3 antibiotics and 25% to at least 2 antibiotics. Results from this study revealed the high prevalence rate of multidrug resistant E. coli isolates. It may suggest that the high resistance of E. coli to antibiotics constitutes a threat not only to poultry industry of Ajmer but also possesses a serious threat to public and animal health with adverse economic implications.