Wild mushrooms

The different solvent extracts of Scleroderma bermudense belongs to the family Sclerodermataceae collected from semi evergreen forest region (13˚51'56.30"N, 75˚03'12.50"E) which is located in Haniya, Hosanagar taluk, Shimoga district, Karnataka was subjected to phytochemical analysis for secondary metabolites and antifungal screening by agar well diffusion method against plant and human pathogenic fungi viz., A. alternate, A. flavus, A. solani, A. tomentosa, C. capsici, C. dematium, C. lindemuthianum, F. oxysporum, F. solani, M. gypseum, T. equinum, T. kanei, C. albicans, C. indicum, C. krusei, C. merdarium, C. zonatum, E. floccosum and T. rubrum. Extracts were found to contain steroids, saponins, glycosides, flavonoids and phenols. The extract also showed significant antifungal activity against C. albicans, C. indicum, C. merdarium, F. oxysporum, C. dematium, T. equinum, A. flavus C. capsici, F. solani, C. kruesi and C. lindemuthianum, whereas least activity showed against A. solani, M. gypseum and does not showed inhibition zone against A. alternate, A. tomentosa. T. kanei, C. zonatum, E. floccosum and T. rubrum. However, the activity was less than the standard Clotrimazole, Fleuconazole, Mancozeb and Captan. The extract shows increasing inhibitory activity with increase in concentration (12.5%-100%).While comparing the solvent studied, petroleum ether and methanol extracts showed highest response in resisting microbial growth than chloroform.