DPPH.

To evaluate the preliminary phytochemical, antioxidant and anti-inflammatory activity of77 Anaphyllum wightii Schott. Bovine Serum Albumin denaturation inhibition assay was used for the evaluation of in-vitro anti-inflammatory activity and DPPH. Free radical scavenging and super oxide scavenging assays were used to assess the antioxidant activity of chloroform and aqueous extracts of the tubers of Anaphyllum wightii Schott. In anti-denaturation study, it was observed that chloroform extract showed greater percentage of inhibition of bovine serum albumin denaturation ie 49.2% whereas aqueous extract showed 38.4% at the 400µg/ml concentration respectively. In case of antioxidant screening also, chloroform extract showed better antioxidant power compared to aqueous extract dose dependently. Among the two extracts evaluated for anti-inflammatory and antioxidant activities chloroform extract of Anaphyllum wightii was found to possess significantly good anti-inflammatory and antioxidant activities and this can be attributed to the presence of alkaloids, saponins, flavonoids and phenolic compounds.

The Antioxidant potential of ethanol extract as well as petroleum ether, chloroform, ethyl acetate and aqueous soluble fractions of Tephrosia purpurea (Linn.) Pers., which is widely used in indigenous system of medicine for different purposes. The ethanol extract as well as petroleum ether, chloroform, ethyl acetate and aqueous soluble fraction were analysed for total phenolics content, total flavonoid content and free radical scavenging activity using DPPH (1,1- diphenyl- 2- picryl hydrazyl) radical. The ethanol extract was found more effective than four different fractions of ethanol extract of Tephrosia purpurea. Total phenolics content and total flavonoid content are found to be highest in ethyl acetate fraction and least in petroleum ether and more or less similar in aqueous and chloroform fractions. In general, the results indicate that the ethanol extract and ethyl acetate fraction are rich in phenolic content and flavonoid content with potent free radical scavenging activity implying their importance to human health. Key Words: Antioxidant, Tephrosia purpurea, DPPH.