Preliminary screening of anaphyllum wightii schott tubers for anti-inflammatory and antioxidant activity

To evaluate the preliminary phytochemical, antioxidant and anti-inflammatory activity of77 Anaphyllum wightii Schott. Bovine Serum Albumin denaturation inhibition assay was used for the evaluation of in-vitro anti-inflammatory activity and DPPH. Free radical scavenging and super oxide scavenging assays were used to assess the antioxidant activity of chloroform and aqueous extracts of the tubers of Anaphyllum wightii Schott. In anti-denaturation study, it was observed that chloroform extract showed greater percentage of inhibition of bovine serum albumin denaturation ie 49.2% whereas aqueous extract showed 38.4% at the 400µg/ml concentration respectively. In case of antioxidant screening also, chloroform extract showed better antioxidant power compared to aqueous extract dose dependently. Among the two extracts evaluated for anti-inflammatory and antioxidant activities chloroform extract of Anaphyllum wightii was found to possess significantly good anti-inflammatory and antioxidant activities and this can be attributed to the presence of alkaloids, saponins, flavonoids and phenolic compounds.