Alloxan

The present study is undertaken to explore the effects of aqueous, alcoholic, chloroform and Petroleum ether extracts of Hibiscus rosa sinensis(HS), Aegle marmelos(AM), and Jatropha curcas(JC) on blood glucose level in both normal and alloxan induced diabetic rats.  In this single dose study, extracts are prepared by Maceration process with different solvents. Extracts of HS, AM, JC  was given once orally in different doses .The effect of extract was studied in normal rats, alloxan (150mg/kg, i.p.) induced diabetic rats and glucose fed normal rats by measuring fasting blood glucose level at different intervals. The extracts exhibited significant (p

The present study was performed to validate floklore claims of leaves of Corchorus trilocularis using ethanol extract for its antihyperglyceamic activity in alloxan induced diabetic rats. Ethanolic extract of C. trilocularis (EtCT) and glyburide were administered orally in alloxan induced diabetic rats. In the acute study, the serum glucose level was estimated at 0, 2, 4, 6 and 24 h after drug administration. The subacute study involved repeated administration of the drugs for 28 days, a serum glucose level estimation at 7, 14, 21 and 28 days. In the OGTT, D-glucose (2.5 g/kg) was administered in diabetic rats half an hour after pre-treatment with EtCT and glyburide. Serum glucose levels were estimated 30 min prior to glucose administration and at 0, 30, 60 and 120 min after glucose loading. In EtCT (400 mg/kg), the onset was 4 h, the peak effect was 6 h but the effect waned at 24 h. In subacute study, repeated administration (once a day for 28 days) of the glyburide and EtCT caused a significant reduction in the serum glucose level as compared to the vehicle treated group. EtCT (400 mg/kg) treatment prevented a decrease in the body weight of the diabetic rats. In the OGTT, EtCT (400 mg/kg) increased the glucose threshold at 60 min after the administration of glucose. The EtCT (400 mg/kg) showed significant antihyperglyceamic activity than EtCT (100 and 200 mg/kg).It can be concluded that ethanolic extract of C. trilocularis has antihyperglyceaamic activity.

Hypoglycemic and hypolipidemic effects of Lepidium sativum (Garden cress) seed powder (3.0g/kg) was evaluated on diabetes and oxidative stress built up in alloxan induced diabetic male Wistar rats. Experimental animals were divided into six groups comprising of six animals each. Animals were supplemented with isoenergic diets (~3600) for a period of 45 days. Hyperlipidemia was induced by feeding high fat high cholesterol diet and diabetes was induced by single injection of alloxan (150mg/kg body weight) intraperitoneally. High fat feeding and alloxan induced diabetes resulted in marked alterations in blood glucose, lipid profile and antioxidant status in blood serum and hepatic tissue of albino rats. Garden cress treated rats showed a significant decrease (p≤ 0.05) in fasting blood glucose levels (FBG); glycosylated haemoglobin (Hb A1C %); lipid profile (total cholesterol (TC), triglycerides (TG) and lipoprotein fractions (Low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C)) with a significant increase in high density lipoprotein cholesterol levels (HDL-C). A significant increase (p≤0.05) in thiobarbituric acid reactive substances (TBARS) levels with concomitant decrease in reduced glutathione (GSH) and antioxidant enzyme activity was also observed in diabetic control and HFHC diet fed experimental rats. Lepidium sativum neutralized the effect and restored the levels. Lepidium sativum seed powder thus lends credence for the prevention and management of diabetes mellitus and related complications. Key words: Lepidium Sativum, Garden Cress, Alloxan, Antihyperglycaemic, Antihyperlipidemic, Oxidative stress, Blood glucose, Lipid peroxidation