Acetonitrile

A new Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method has been developed for estimation of Paracetamol and Alprazolam in bulk and tablet dosage forms using UV-detector. A RP Cell Pack C18 column (250 mm × 4.6 mm, 5 µ particle size) using acetonitrile and water (80:20 % V/V) as mobile phase by maintaining flow rate of 1 mL/min at 236 nm as detection wavelength. The peaks were eluted at 4.8 and 6.2 mins for Paracetamol and Alprazolam, respectively. The method was validated in accordance with ICH guidelines, the linearity curve for Paracetamol was obtained over the range of 50-175 µg/mL, and it was found to be linear with y = 1961x + 9226 (r2 = 0.999). The linearity curve for Alprazolam was obtained over the range of 0.25-1.5 µg/mL and was found to be linear with y =23328x + 939.3 (r2 = 0.998). The percentage recoveries were found to be 99-101% and 99-102%, respectively. The system suitability parameters such as number of theoretical plates and tailing factor were found to be 7242, 1.56 for PAR and 6755, 1.15 for ALP. Hence the developed RP-HPLC method was found to be simple, accurate, economical, rapid and can be applied for routine analysis of these drugs in their combined formulations.

This present study was undertaken with an objective to develop & validate a simple, precise, cost effective, sensitive & fast RP- HPLC method for the analysis of Ranolazine. A Shimazu HPLC system with Luna 5µm C18 is employed for the analysis using Methanol: Acetonitrile(50:50, v/v) as mobile phase. Signal from Ranolazine is detected at 227nm by UV Spectrophotometer. The total retention time was 5 min with a flow rate of 1.0 ml/min. % 0f RSD values for precision is found to be 0.798%.The limits of detection (LOD) and quantification (LOQ) were 0.616 and 1.86, respectively. As per ICH guidelines the proposed method is fully validated and found to be linear over a workable drug concentration, accurate, precise and robust. This fast, simple and inexpensive method is suitable for research laboratories & also for quality control analysis in pharmaceutical industries.

The present study was undertaken to develop a validated stability-indicating liquid chromatography method for estimating Everolimus in commercial tablet dosage forms. Chromatographic separation was achieved on Kromasil C18column(100mm x 4.6 mm, 5m) with mobile phase containing potassium dihydrogen orthophosphate buffer and acetonitrile taken in the ratio 75:25 v/v. The pH was adjusted to 3.0 with dilute orthophosphoric acid at a flow rate of 1.0 mL/min and the eluent was monitored at 270 nm. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to specificity, precision, linearity, accuracy and robustness. Linearity range was found to be between 5-30 ppm and the linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating that the accuracy and precision of the method are good. Statistical analysis proved that the method was precise, reproducible, selective, specific, and accurate for analysis of Everolimus. All the degradation products formed during forced degradation studies were well separated from the analyte  peak.

Meloxicam has been analyzed in a number of ways but the methods proved to be slow and tedious due to the involvement of buffers, maintaining of pH and lengthy process of HPLC or potentiometric titration. Our aim was development and validation of a new, fast, simple and inexpensive method for the analysis of Meloxicam by UV visible spectrophotometry. A new solvent system consisting of Acetonitrile and Methanol (70:30) was formulated by trial and error method. Different concentrations of meloxicam were made in this solvent system and were analyzed by UV visible spectroscopy. The absorbance was studied over the range of 240 to 400nm. The obtained results were used to study and calculate various validation parameters of this technique.  The calibration curve was found to be linear over the range of 0.1 to 0.6ug/ml with linear coefficient of 0.999. The precision was calculated to be equivalent to 0.251%. The LOD and LOQ of this method were found to be around 0.006ug/ml and 0.02ug/ml respectively. The system showed accuracy over the range of 90-100%. This method proved to be a good alternative to existing reported methods as it showed sensitivity, accuracy as well as reproducibility with low cost and time.

A simple, rapid and accurate high performance liquid chromatography method is described for determination of rupatadine fumarate from its active pharmaceutical ingredients. The separation of drug was achieved on Inertsil ODS-3 C18 (250 X 4.6 mm) 5µ column. The mobile phase was a mixture of acetonitrile and methanol (80:20 v/v) : buffer of pH  4.5 [70:30 % v/v]. The buffer of pH 4.5 was prepared from 0.01 M ammonium acetate and pH was adjusted with dilute acetic acid. The detection was carried out at wavelength 250 nm. The mixture of water: acetonitrile (30:70 % v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze rupatadine fumarate from pharmaceutical formulation.