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The present study was undertaken to determine the forced degradation of Pyrazinamide, performed by various conditions such as acid, alkali, oxidation, thermal and photolytic. The study includes both Pyrazinamide in bulk and tablet formulation. The study based on available guidelines and main reference .Pyrazinamide has a Pyrazine nucleus. It is easily hydrolyzed by acid and alkali. The assay value of degraded products measured by intraday (30mins, 60mins, 90mins) and interday (1st, 3rd, 5th day) by UHPLC. Extensive degradation was observed in alkali hydrolysis method, and the degraded products were analysed by using UHPLC. At 90mins of intraday study using 0.1M NaOH, the degradation assay value of bulk and formulation were found to be 84.50% and 83.40% respectively. Intraday study, the degradation of bulk and formulation was observed on 1st day with the assay value of 23.62% and 25.42% respectively. However complete degradation of Pyrazinamide was observed on 3rd day and 5thday. It was determined that Pyrazinamide was found to be extremely unstable under alkali condition.

The present study was undertaken to develop a validated stability-indicating liquid chromatography method for estimating Everolimus in commercial tablet dosage forms. Chromatographic separation was achieved on Kromasil C18column(100mm x 4.6 mm, 5m) with mobile phase containing potassium dihydrogen orthophosphate buffer and acetonitrile taken in the ratio 75:25 v/v. The pH was adjusted to 3.0 with dilute orthophosphoric acid at a flow rate of 1.0 mL/min and the eluent was monitored at 270 nm. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to specificity, precision, linearity, accuracy and robustness. Linearity range was found to be between 5-30 ppm and the linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating that the accuracy and precision of the method are good. Statistical analysis proved that the method was precise, reproducible, selective, specific, and accurate for analysis of Everolimus. All the degradation products formed during forced degradation studies were well separated from the analyte  peak.

A reverse phase high performance liquid chromatographic method was developed for the determination of Mirabegron in bulk and Pharmaceutical dosage form. The separation was effected on a Waters ODS C18 column (150 mm x 3.9 mm;5µ) using a mobile phase mixture of buffer and acetonitrile in a ratio of 50:50 v/v at a flow rate  of 1ml/min. The detection was made at 249 nm. The retention time of Mirabegron was found to be 2.502 min.  Calibration curve was linear over the concentration range of 6.25-37.5 µg/ml of Mirabegron. The propose method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.