Tablets

A simple Reverse phase liquid chromatographic method has been developed and subsequently validated for estimation of lansoprazole in tablet dosage form. The separation was carried out using a mobile phase consisting of Methanol and 0.1% OPA (Ortho Phosphoric Acid) in the ratio of 70:30. The column used was C18 and 250 mm length with flow rate of 1.2 ml / min using UV detection at 285nm. The described method was linear over a concentration range of 10-50 μg/ml for the assay of Lansoprazole. The retention time of Lansoprazole was found to be 6.6 min, and all the results of analysis were validated statistically. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Lansoprazole in tablet dosage form and in its pharmaceutical dosage forms.

A simple, sensitive, rapid, accurate and precise spectrophotometric method has been developed for estimation of Glimepiride in bulk and tablet dosage forms. The zero order spectra shows maximum absorbance at 249 nm. Calibration graph was found to be linear over the concentration range of 5-30 μg/ml. Results of analysis were validated for precision, range, linearity, interference study and recovery studies, The method can be adopted in its routine analysis.

An accurate, rapid, specific and stability indicating ultra performance liquid chromatographic method was developed and validated for the simultaneous estimation of Verapamil and Trandolapril in a combined pharmaceutical dosage form. The chromatographic separation was attained on Phenonemex Luna C18 (4.0 x 100 mm, 2.6mm) column by isocratic mode with the mobile phase components as 0.03M monobasic potassium phosphate buffer (pH6.5) and acetonitrile (70: 30v/v) at a flow rate of 1.0 mL/min and quantified at 210 nm. The average retention times for Trandolapril and Verapamil were 0.60 and 1.14 min, respectively. The UPLC method proposesoutstanding separation of two drugs with a good resolution of greater than 2.0 and tailing factor less than 2.0 with a run time of 3 minutes. The method shows linearity over the concentration range of 9-45 µg/mL for Verapamil and 0.1-0.5 µg/mL for Trandolapril with a correlation ≥ 0.999. The method is accurate with recoveries in the range of 98.0 -101.0% and precise with %RSD value lesser than2.0% for both the drugs. This method is very fast, cost saving, accurate and specific for the assay of commercially available tablets.

An accurate and precise high performance liquid chromatographic method was developed for quantitative estimation of bosentan in tablet dosage forms. Chromatographic separation of the drug was achieved on a Kromasil C18 column (150 x 4.6 mm; 5µ) by eluting with a mobile phase consisting of phosphate buffer (pH 4.0) and acetonitrile (30:70 v/v) at a flow rate of 1.0 mL/min. The drug in the eluate was monitored by U V detection at 270 nm. The retention time obtained for the drug was 3.54 min. The calibration curve plotted was linear over the range of 25-175 µg/mL of the drug. The validation of the method was done following the ICH guidelines. The proposed method could be applied for determination of bosentan in its tablet dosage forms without any interference from excipients. The method is suitable for routine quality control analysis of bosentan formulations.

A new simple fast accurate and economical reverse phase high performance liquid chromatographic method was developed for the determination of Ramipril and Olmesartan in bulk and tablet dosage form. The separation was eluted on a Inertsil C8 column (100 mm x 4.6 mm; 5µ) using a mobile phase mixture of mixed phosphate buffer 6.8 and acetonitrile in a ratio of 65:35 v/v at a flow rate of 1.0ml/min. The detection was made at 219 nm. The retention times were 2.28min for Ramipril and 3.76min for Olmesartan. Calibration curve was linear over the concentration range of 2.5-15 µg/ml for Ramipril and 10 to 60 µg/ml for Olmesartan. The propose method was validated as per the ICH guidelines parameters like Linearity, specificity, precision, accuracy, robustness and ruggedness. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.

An accurate, precise and reproducible high performance liquid chromatographic method was developed for the simultaneous estimation of lamivudine, zidovudine and nevirapine in pharmaceutical dosage forms. Phenomenex C18 column (250 x 4.6 mm; 5µ) was employed for the separation of drugs. A mixture of 0.02 M trichloroacetic acid (6.8 pH) and methanol in the ratio of 40:60 v/v was used as the mobile phase and pumped at a flow rate of 1ml/min. The detection wavelength was set at 265 nm. The linearity of quantification was observed in the range of 7.5-112.5, 10-150 and 15-225 μg/ml for lamivudine, zidovudine and nevirapine respectively. The proposed method was validated according to ICH guidelines. The method was found to be suitable for simultaneous and accurate determination of these drugs in tablet dosage forms without any interference from the excipients.

An accurate, precise and reproducible high performance liquid chromatographic method was developed for quantitative estimation of telmisartan in bulk drug samples and tablet dosage forms. Chromatographic separation of the drug was achieved on a Kromasil C18 column (150 x 4.6 mm; 5µ) using a mixture of phosphate buffer (pH 4.0) and acetonitrile (40:60 v/v) as the mobile phase at a flow rate of 1.0 mL/min. Under optimized conditions, the retention time of the drug was found to be 2.887 min. Good detecting sensitivity for the analyte was observed at 224 nm. The quantitation calibration curve for the drug was linear over the range of 20-60 µg/mL. The performance of the proposed method was validated as per ICH guidelines. The method was proved to be suitable for the estimation of telmisartan in tablet dosage forms. Key words: Telmisartan, Estimation, Tablets, HPLC.

A reverse phase high performance liquid chromatographic method was developed for the determination of Mirabegron in bulk and Pharmaceutical dosage form. The separation was effected on a Waters ODS C18 column (150 mm x 3.9 mm;5µ) using a mobile phase mixture of buffer and acetonitrile in a ratio of 50:50 v/v at a flow rate  of 1ml/min. The detection was made at 249 nm. The retention time of Mirabegron was found to be 2.502 min.  Calibration curve was linear over the concentration range of 6.25-37.5 µg/ml of Mirabegron. The propose method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.

  Nothing in this world is stable and ever accepted. Change is the requirement of nature for the sake of adaptability. However, the pharmaceutical world is also not far off from this change. Technical advancement in pharma world also leads to the development of new dosages forms. This leads to the replacement of the older dosages forms with the newer once. But for the tablet dosages forms this replacement is substituted with modifications. On the top of it the availability of numerous evaluation parameters provides these new modifications in tablets a clear cut demonstration idea.