Development of UV spectroscopic technique for analysis of Meloxicam raw material and pharmaceutical dosage forms

Meloxicam has been analyzed in a number of ways but the methods proved to be slow and tedious due to the involvement of buffers, maintaining of pH and lengthy process of HPLC or potentiometric titration. Our aim was development and validation of a new, fast, simple and inexpensive method for the analysis of Meloxicam by UV visible spectrophotometry. A new solvent system consisting of Acetonitrile and Methanol (70:30) was formulated by trial and error method. Different concentrations of meloxicam were made in this solvent system and were analyzed by UV visible spectroscopy. The absorbance was studied over the range of 240 to 400nm. The obtained results were used to study and calculate various validation parameters of this technique.  The calibration curve was found to be linear over the range of 0.1 to 0.6ug/ml with linear coefficient of 0.999. The precision was calculated to be equivalent to 0.251%. The LOD and LOQ of this method were found to be around 0.006ug/ml and 0.02ug/ml respectively. The system showed accuracy over the range of 90-100%. This method proved to be a good alternative to existing reported methods as it showed sensitivity, accuracy as well as reproducibility with low cost and time.