Velliyur Kanniappan Gopalakrishnan

The aim of the present study was to investigate the chemical composition of Spirulina subjected to different solvent extraction (acetone, methanol and ethyl acetate) by using soxhlet methodology and the extracts were analysis by Gas chromatography coupled with mass spectrophotometer (GC-MS) using DB-5 capillary column. During GC-MS analysis, it was observed that fatty acid components are present in their extracts. In acetone extract, totally forty compounds were identified and heptacosane (17.25%), hexacosane (17.04%), heneicosane (14.47%), pentacosane (13.22%) and Nonacosane (11.29%) as major components. Thirty five compounds were identified in methanol extract and the major compounds are n-Hexadecanoic acid (19.9%), Cyclononasiloxane octadeca methyl (7.81%) and phytol (6.53%). Thirty components were identified in ethyl acetate extract and dodecane (17.35%), heptadecane (15.31%), sufurous acid butyl heptadecyl ester (11.31%) and n-hexadecanoic acid (4.26%) most abundant in ethyl acetate extract.

Most of the synthetic drugs which induce side effect during overdose. The natural compounds are playing important role to reducing side effects of the synthetic drugs and also increase the immunity of recipients. The objective of the present study is to investigate the compatibility between Paracetamol as a synthetic compound with Spirulina as a natural compound.  The Paracetamol and Spirulina mixture were prepared in different ratio 1:1 (w/w) (Paracetamol: Spirulina), 1:2 (w/w) (Paracetamol: Spirulina) and 2: 1 (w/w) (Paracetamol: Spirulina). These samples were stored at different temperature (5 ˚C, 15 ˚ C, 30 ˚ C and 40 ˚ C) for one month. For every week samples were taken in the mixtures and the quantification of Paracetamol by using reversed phase liquid chromatography and estimation of chlorophyll A, B, total chlorophyll and total carotenoids in the mixture by using UV spectrophotometer. There was no significant loss of Paracetamol, chlorophyll, and carotenoids in their mixture. Both Paracetamol and Spirulina were stable under different temperature. Therefore it conclude that Paracetamol and Spirulina were compatible when mixed in a ratio of 1:1(w/w), 1:2 (w/w) and 2:1 w/w)