UV spectrophotometer

Albendazole (ALB), chemically known as methyl [5-(propylthio)-1H-benzimidazol-2-yl] carbamate is widely used as an anthelmintic having a wide spectrum of activity. Numerous numbers of analytical methods are there for the simultaneous estimation of bulk and in formulation, such as spectrophotometry and liquid chromatography. As the UV spectrophotometric method is rapid, simple, accurate and economical, the method has been developed for the assay of the albendazole in pharmaceutical formulation. The wavelengths selected for the method were at 291 nm. The results of analysis have been validated by recovery studies as per ICH guidelines. The developed method was rapid, simple, accurate and economical and it can be used for routine quality control analysis. It showed absorption maxima at 291 nm in analytical grade DMF. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity.

Most of the synthetic drugs which induce side effect during overdose. The natural compounds are playing important role to reducing side effects of the synthetic drugs and also increase the immunity of recipients. The objective of the present study is to investigate the compatibility between Paracetamol as a synthetic compound with Spirulina as a natural compound.  The Paracetamol and Spirulina mixture were prepared in different ratio 1:1 (w/w) (Paracetamol: Spirulina), 1:2 (w/w) (Paracetamol: Spirulina) and 2: 1 (w/w) (Paracetamol: Spirulina). These samples were stored at different temperature (5 ˚C, 15 ˚ C, 30 ˚ C and 40 ˚ C) for one month. For every week samples were taken in the mixtures and the quantification of Paracetamol by using reversed phase liquid chromatography and estimation of chlorophyll A, B, total chlorophyll and total carotenoids in the mixture by using UV spectrophotometer. There was no significant loss of Paracetamol, chlorophyll, and carotenoids in their mixture. Both Paracetamol and Spirulina were stable under different temperature. Therefore it conclude that Paracetamol and Spirulina were compatible when mixed in a ratio of 1:1(w/w), 1:2 (w/w) and 2:1 w/w) 

The neem tree has been known for its unique properties both in improving human health and against insects. Azadirachtin is a tri terpenoid limonoid obtained from various parts of the neem. Phosphatidyl choline is the most important phospholipid which helps in crossing the lipid barrier of the cell membrane and thus helps in easy absorption of the compound/drug. In the present work we are combining the isolated Azadirachtin and PC to form a complex, which can enhance the bio availability and easy absorption of the compound/drug. First of all, Azadirachtin is extracted from the dried neem kernel powder in a Soxhlet extractor using Di-Chloro methane as a solvent. Phosphatidyl choline is extracted and isolated from soya lecithin oil through reflux, using Methanol + Sodium hydroxide as solvent. The TLC, UV, HPLC, FTIR and DSC reports indicate the separation of the compounds from their crude forms. Azadirachtin and Phosphatidyl choline separated are refluxed together for 2 hours and dried in a Rota evaporator to form a 'Complex'. The absorbance's of Azadirachtin, Phosphatidyl choline and the Complex are measured at 220, 230 and 217nm. The qualitative analysis of Azadirachtin, Phosphatidyl choline and the Complex (1:1) were carried out by HPLC, on a C-18 column. Aceto nitryl: Methanol: 1% Triethyl amine pH 4 (60:40:1) was used as mobile phase at a flow rate of 1ml/min, at 210nm.The isolation of Phosphatidyl choline from soya lecithin oil used is a new method and the formation of Complex is an innovative work.