Rahul Ghongade

The biodiesel is quite similar to conventional diesel fuel in its physical characteristics and can be used alone or mixed in any ratio with petroleum based diesel fuel in most existing modern four-stroke combustion ignition diesel engines with very few technical adjustments or no modification. Biodiesel as a neat can be used as a direct substitute for petro diesel and is technically called B100.The reaction is catalyzed by NaOH making this process economically viable for the industrial scale production of biodiesel. Biodiesel is an excellent product as it is environmentally friendly. Chromatogram GC-MS analysis showed 12 peaks were detected as methyl esters of fatty acids in Biodiesel coconut oil but there is only one a prominent peak that has great intensity.

The aim of the study was to assess Pharmacognestic study of crude plant extract of Rivea hypocrateriformis and was carried out to characterize the chemical composition of some constituents by GC-MS analysis. Different solvent extracts (aqueous, methanolic, chloroform, ethyl acetate and DMSO) of plant R. hypocrateriformis leaves were assessed for in vitro antimicrobial activity assay by disc diffusion method furthermore antioxidant assay was carried out DPPH free radical scavenging activity, Phytochemical screening was carried out by ‘guide to modern techniques of plant analysis” and GC-MS analyses were performed to identify the constituents present in the plant that stand behind such activities. Due to higher polarity, DMSO extract show revealed presence of maximum phytochemical composition susceptibility as well as methanol and chloroform shows average amount of phytoconstituents. The antibacterial screening is the major of the inhibition hollow observed in inhibition zone. The highest inhibition zone was observed in DMSO extract against each bacterial strain. Where E. coli shows mid active zone inhibition and S. aureus show less, IC50 value of the sample was found to be moderate as compared to standard and the eight compounds were identified in R. hypocrateriformis leaf extract by GCMS analysis. R. hypocrateriformis plant had considerable major chemical composition present in crude extract. Due to presence of major chemical components make it seems to be important for medical purposes and plant contains Potential antibacterial components that may be useful for evolution of pharmaceutical for the therapy of ailments. Also plant extracts can be used for the treatment of infections caused by the strains of the test bacterial organisms.