M. Umadevi

A new series transition metal complexes of Cu(II), Co(II), Ni(II) and Zn(II)  complexes have been synthesized from 2-(4-amino-5-mercapto-4H-1,2,4-triazol-3-yl)phenol (AMPT). The nature of bonding and the geometry of the complexes have been deduced from elemental analyses, magnetic susceptibility, infrared, electronic, 1H NMR, 13C NMR, and EPR spectral studies. The redox behavior of copper complexes was studied by cyclic voltammetry.  The second harmonic generation (SHG) efficiency was measured by Kurtz and Perry method. The ligand and their metal complexes were screened by Well diffusion method. The interaction of complexes with CT- DNA was investigated by viscosity measurement. Results suggest that all the complexes bind to DNA via an intercalative mode. The DNA cleavage ability of all the complexes was examined on calf thymus (CT-DNA) plasmids using gel electrophoresis experiment in presence of H2O2. From the results it is concluded that all the complexes cleave DNA.

Three novel Mannich bases 5-[(4-chlorophenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione :CBzBAPz(NM), 5-[(4-nitrophenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione: NBzBAPz(NM) and 5-[(phenyl)-(4-methylpiperazin-1-yl)-methyl]-pyrimidine-2,4,6-trione: BzBAPz(NM) are prepared by the condensation of N-methyl piperazine with p-chlorobenzaldehyde / p-nitro benzaldehyde / benzaldehyde and barbituric acid and  characterized by physico-chemical,  spectroscopic, and powder X-ray diffraction studies. Further, Cu (II), Cd (II) and Pd (II) complexes of titled ligands are synthesized and characterized. Based on the experimental results, the chelating (bidentate) behavior of the ligands is accessed. The X-band e.p.r.spectrum, electrochemical studies of the Cu (II) complex have been carried out and discussed. Three novel Mannich bases and their complexes exhibit comparable potential cytotoxicity against breast cancer cell line (MCF-7), particularly, strong cytotoxicity is observed in the case of palladium. Morphological features of cells used for the study is photographed. Use of fluorochromesacridine orange and ethidium bromide reveals apoptotic morphological features.