Devprakash

The Antioxidant potential of ethanol extract as well as petroleum ether, chloroform, ethyl acetate and aqueous soluble fractions of Tephrosia purpurea (Linn.) Pers., which is widely used in indigenous system of medicine for different purposes. The ethanol extract as well as petroleum ether, chloroform, ethyl acetate and aqueous soluble fraction were analysed for total phenolics content, total flavonoid content and free radical scavenging activity using DPPH (1,1- diphenyl- 2- picryl hydrazyl) radical. The ethanol extract was found more effective than four different fractions of ethanol extract of Tephrosia purpurea. Total phenolics content and total flavonoid content are found to be highest in ethyl acetate fraction and least in petroleum ether and more or less similar in aqueous and chloroform fractions. In general, the results indicate that the ethanol extract and ethyl acetate fraction are rich in phenolic content and flavonoid content with potent free radical scavenging activity implying their importance to human health. Key Words: Antioxidant, Tephrosia purpurea, DPPH.

  In the present research a simple, accurate, precise and cost effective UV-Vis spectrophotometric method for the estimation of Metformin, in bulk and pharmaceutical dosage form was illustrated. The absorption maxima of the drug was found to be 233 nm in 0.1 N HCl: Distilled water (27:75). A linear response was observed in the range of 5-10 µg/ml with a regression coefficient of 0.999. Validation parameters were carried out as per the guidelines of International Conference for Harmonization. This method can be used in the industries for determination of Metformin to analyze the quality of formulation without interference of the excipients.

  A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Glibenclamide in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 5-30µg/ml with correlation coefficient of 0.999 in acetronitrile: 0.2M NaOH (20:80). Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines. Key words: Glibenclamide, UV- Spectrophotometry, recovery, accuracy.

  A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Clopidogrel bisulfate in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 25-50 µg/ml with correlation coefficient of 0.999 in 0.1 N HCl. Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines.  

  In the present research a simple, accurate, precise and cost effective UV-Vis spectrophotometric method for the estimation of Clobazam, in bulk and pharmaceutical dosage form was illustrated. The absorption maxima of the drug was found to be 230 nm in methanol: water (1:1). A linear response was observed in the range of 6- 16 µg/ml with a regression coefficient of 0.999. Validation parameters were carried out as per the guidelines of International Conference for Harmonization. This method can be used in the industries for determination of Clobazam to analyze the quality of formulation without interference of the excipients.   Key words: Clobazam, Anti-epileptic, λmax, ICH, UV-Vis spectroscopy.