accuracy.

A Novel, simple, accurate, and precise Area under curve spectroscopic method was developed and validated for the estimation of Diltiazem Hydrochloride in Pure and pharmaceutical dosage forms and has an absorption maximum between 232-242nm in 0.1M Sulphuric acid. The stock solution was made to produce 1000 µg/ml with 0.1M Sulphuric acid. The linearity was found in the concentration range of 1-3.5 µg/ml. The correlation coefficient was found to be 0.9999. The regression equation was found to be Y=0.1013x+0.002. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation and ruggedness. The limit of detection and limit of quantitation for estimation of Diltiazem Hydrochloride was found to be 0.01066µg/ml and 0.1066µg/ml, respectively. Recovery of Diltiazem Hydrochloride was found to be in the range of 100.02-101.68 %. The %RSD values were less than 2. The method has been validated according to ICH guidelines. The Proposed method was successfully applied for the quantitative determination of Diltiazem Hydrochloride in pharmaceutical dosage forms. Keywords: Diltiazem Hydrochloride, Area under curve Spectroscopy, 0.1M Sulphuric acid, accuracy.  

A simple, economic, selective, precise and accurate High-Performance liquid Chromatographic method used for the estimation of Adenosine in bulk drug. The mobile phase used was of Mixture of Acetonitrile and water in the proportion 5:95 respectively. This Mobile phase was allowed to flow at rate of 0.8ml/min. And this was found to give a sharp peak of Adenosine at a retention time of 3.78 min. Analysis of HPLC for Adenosine was carried out at a wavelength of 256 nm. Linear regression analysis data for the Calibration curve showed a good linear relationship, in concentration range of 50-100ppm and regression coefficient 0.991. The linear regression equation was Y=71258× the developed method was employed with a high degree of precision and accuracy for the analysis of adenosine. The inter and intraday variation was less than 2%. The mean recovery of the drug was 99.39%. The proposed method is simple, fast, accurate, and reproducible hence, it can be applied for routine quality control analysis of Adenosine.

  A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Glibenclamide in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 5-30µg/ml with correlation coefficient of 0.999 in acetronitrile: 0.2M NaOH (20:80). Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines. Key words: Glibenclamide, UV- Spectrophotometry, recovery, accuracy.

  A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Clopidogrel bisulfate in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 25-50 µg/ml with correlation coefficient of 0.999 in 0.1 N HCl. Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines.