UV- Spectrophotometry

Hypertension (HT) is a very common disorder, particularly for past middle age. It is not a disease in itself, but is an important risk factor for cardiovascular mortality and morbidity. For improved treatment of hypertension, Telmisartan and Benidipine HCl is the newer combination in market, this combination was developed to improve medication for Stage II hypertension. The aim of this review is to focus on comprehensive update of different analytical methods used for estimation of anti-hypertensive drugs like Telmisartan and Benidipine HCl for the treatment of hypertension. This review delivers a detail description on different analytical methods like UV and RP-HPLC for Telmisartan and Benidipine HCl individually and combination with other drugs. For this review, data searches were conducted by scientific papers in the literature as well as in official compendium. All reported methods are found to be simple, accurate, economic, precise and reproducible in nature.

  A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Glibenclamide in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 5-30µg/ml with correlation coefficient of 0.999 in acetronitrile: 0.2M NaOH (20:80). Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines. Key words: Glibenclamide, UV- Spectrophotometry, recovery, accuracy.

  A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Clopidogrel bisulfate in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 25-50 µg/ml with correlation coefficient of 0.999 in 0.1 N HCl. Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines.