A.Suganthi

The objective of the study was to determine the anticancer efficacy of M. maderaspatana fruit extract against Michigan Cancer Foundation-7 (MCF-7). Different concentration (10, 20, 30, 40, and 50 μg/ml) of methanol extract of M. maderaspatana fruit were used to assess the in vitro cytotoxic effect using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Identification and quantification of beta carotenoid in HPLC. The various concentrations of crude methanol extract (10, 20, 30, 40, and 50 μg/ml concentration) of M. maderaspatana fruit were performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against Michigan Cancer Foundation-7 (MCF-7)   with an inhibit cell growth by 50% (IC50) of 32 ± 1.0 μg/ml. The βeta- carotene content through spectrophotometry and high performance liquid chromatography HPLC analysis showed that 88ppm of betacarotene present in M.maderspantana fruits.  The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the M.maderspantana extract indicates that has anticancer activity against Michigan Cancer Foundation-7 (MCF-7)   cancer cell lines. The present study concluded that the methanol extract of M.maderspantana possess potent cytotoxic activity.

Euphorbia Hirta belonging to the Euphorbiaceae family contains more amounts of phenolic compounds and flavonoids which are responsible for the main pharmacological actions like anti-oxidant, anti-inflammatory, anti-dengue and anti-cancer. Considering the current importance of these ingredients, an attempt has been made for the simultaneous estimation of gallic acid, rutin and quercetin from euphorbia hirta by successive extraction involves the use of solvents in an order of increasing polarity in soxhlet extractor at 30-45 °C using 800 ml solvents for 5 hours in increasing polarity to isolate the active constituents without other interferences. Hence we proposed to develop easy, rapid, accurate, precise and reliable analytical HPTLC method for the standardization of Euphorbia hirta(L) using gallic acid, rutin and quercetin as phytochemical markers from its methanolic extract and herbal capsule formulation. The separation was performed on TLC aluminum Plates precoated with silica gel 60F254, good separation was achieved in the mobile phase of butyl acetate: 1,4-dioxane (5:5% v/v) and densitometric determination of gallic acid, rutin and quercetin was carried out at 266nm.The linear regression data showed a good linearity in the concentration range of 136-748ng/spot of gallic acid, rutin and quercetin with a good correlation coefficient of 0.9989. Limit of detection was found to be 102 ng/spot of gallic acid,17ng/spot of rutin and 68ng/spot of quercetin. The limit of quantification for the estimation of gallic acid, rutin and quercetin was found to be 136ng/spot.

A simple, specific, sensitive, precise and stability-indicating reversed phase high performance liquid chromatographic method was developed and validated for the simultaneous determination of thiocolchicoside and aceclofenac, using a RP-18 column and a mobile phase composed of 0.1% trifluoroacetic acid: acetonitrile (45:55). The retention time of thiocolchicoside and aceclofenac were found to be 2.35 and 13.2 min, respectively. Linearity was established for both thiocolchicoside and aceclofenac in the range of 0.08-0.8 and 1-10 µg/ml. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, and photolytic degradation. The degradation studies indicated that both thiocolchicoside and aceclofenac were susceptible to acid, alkaline and neutral hydrolysis. The degradation products of thiocolchicoside and aceclofenac were well resolved from the pure drugs with significant differences in the retention time values. This method can be successfully employed for simultaneous quantitative analysis of thiocolchicoside and aceclofenac in bulk drugs and formulations.

A simple validated high performance thin layer chromatographic method was developed for the determination of Aceclofenac in presence of its degradant. Separation of Aceclofenac from the degradant could be achieved using aluminium backed silica gel 60 F254 plate with toluene: ethyl acetate: glacial acetic acid, (6:4:0.02v/v) as mobile phase. Densitometry analysis was carried out at 282 nm. The method showed high sensitivity with good linearity over the concentration range of 0.5 – 4 µg/spot. The method was successfully applied to the analysis of pharmaceutical formulation containing Aceclofenac with excellent recovery. The LOD and LOQ were found to be 0.1 and 0.5 µg/spot. Aceclofenac was subjected to hydrolytic, oxidative, thermal and photolytic degradation. It was found that the drug was highly susceptible to acid hydrolysis. Kinetic investigation of the drug followed pseudo-first order reaction. From the Arrhenius plot the activation energy was found to be 13.19 kcal/mole. Statistical analysis revealed that the developed method is accurate and reliable. Hence it can be used for routine quality control analysis of Aceclofenac in tablet formulation.