Fruit

The objective of the study was to determine the anticancer efficacy of M. maderaspatana fruit extract against Michigan Cancer Foundation-7 (MCF-7). Different concentration (10, 20, 30, 40, and 50 μg/ml) of methanol extract of M. maderaspatana fruit were used to assess the in vitro cytotoxic effect using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Identification and quantification of beta carotenoid in HPLC. The various concentrations of crude methanol extract (10, 20, 30, 40, and 50 μg/ml concentration) of M. maderaspatana fruit were performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against Michigan Cancer Foundation-7 (MCF-7)   with an inhibit cell growth by 50% (IC50) of 32 ± 1.0 μg/ml. The βeta- carotene content through spectrophotometry and high performance liquid chromatography HPLC analysis showed that 88ppm of betacarotene present in M.maderspantana fruits.  The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the M.maderspantana extract indicates that has anticancer activity against Michigan Cancer Foundation-7 (MCF-7)   cancer cell lines. The present study concluded that the methanol extract of M.maderspantana possess potent cytotoxic activity.

Dadima (Pomegranate) is one of the most frequently used drug as a part of medicine and food in Ayurved having varied effects on the human body. Dadima is used in Mukharoga (diseases of mouth), Shukra daurbalya (sexual debility), Samanya daurbalya (general debility), Aruchi (tastelessness), Amlapitta (acidity), etc. and is having effects like Daha shamaka (reduces burning sensation), Jwaraghna (antipyretic), Shukrala (increases semen), Pachana (digestive), Balya (general tonic) and Medhya (brain tonic). Till date the detailed pharmacognostical aspects of Dadima i.e its fruit bark, false septa, seed, powder microscopy including its histochemical evaluation was not found reported systematically. The present study was conducted on the fruit of Dadima (Punica granatum Linn.) for its pharmacognostical characters. The microscopic characters of the fruit show epidermis covered with thick cuticle, single layered epicarp, paranchyma cells filled with prismatic crystals, simple and compound starch grains and oil globules, well developed parenchymatous cells, fibres, schleroids, stone cells, prismatic crystals etc.