Thiocolchicoside

A simple, precise, and accurate UV spectrophotometric method was developed and validated for the simultaneous estimation of Lornoxicam and Thiocolchicoside in bulk and pharmaceutical formulations. The method employs methanol as solvent, and absorbance was measured at the respective wavelengths where both drugs showed maximum absorbance with minimal interference. Calibration curves were linear within the concentration ranges of 4–20 µg/mL for Lornoxicam and 5–25 µg/mL for Thiocolchicoside, with correlation coefficients (R²) of 0.9992 and 0.9995, respectively. Validation was carried out according to ICH Q2(R1) guidelines, including parameters such as linearity, accuracy, precision, LOD, LOQ, robustness, and ruggedness. Recovery studies at 50%, 100%, and 150% levels showed recoveries between 98.7–100.1%, indicating high accuracy. The proposed method is suitable for routine quality control of combined dosage forms of Lornoxicam and Thiocolchicoside.

A new validated RP – HPLC method was developed for the simultaneous determination of Thiocolchicoside and Ketoprofen in combined dosage form. The method developed produced high sensitivity, precision and accuracy. An isocratic C18 (Inertsil ODS, 250 x 4.6 mm, 5µ) column was used with mobile phase of composition Acetonitrile: Phosphate buffer (70: 30 at pH 4.6) at a flow rate of 1.0 mL/min with UV detection at 258.2 nm for separating Thiocolchicoside and Ketoprofen. The retention time of Thiocolchicoside and Ketoprofen were 2.4 min and 3.5 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines. Linearity for Thiocolchicoside and Ketoprofen were found in the range of 2.0 – 12.0 µg/ml and 6.2-38.75 µg/ml, respectively. The percentage recoveries for Thiocolchicoside and Ketoprofen ranged from 99.35- 100.21% and 98.66-99.29 %, respectively. The proposed method could be used for routine analysis of Thiocolchicoside and Ketoprofen in their combined dosage forms. All the proposed methods for Thiocolchicoside and ketoprofenare simple, selective, reproducible and specific with good precision and accuracy. The method was proved to be superior to most of the reported methods. These proposed methods for estimation of selected drugs were successfully applied either in tablet dosage form. More over the low solvent consumption along with short retention time of 2.4 and 3.5 for both Thiocolchicoside and Ketoprofen to be cost effective when compared to other developed method shown in literature reviews. The proposed method can be used as alternative methods to the reported ones for the routine determination of selected drugs under the study in tablet dosage form

A simple, specific, sensitive, precise and stability-indicating reversed phase high performance liquid chromatographic method was developed and validated for the simultaneous determination of thiocolchicoside and aceclofenac, using a RP-18 column and a mobile phase composed of 0.1% trifluoroacetic acid: acetonitrile (45:55). The retention time of thiocolchicoside and aceclofenac were found to be 2.35 and 13.2 min, respectively. Linearity was established for both thiocolchicoside and aceclofenac in the range of 0.08-0.8 and 1-10 µg/ml. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, and photolytic degradation. The degradation studies indicated that both thiocolchicoside and aceclofenac were susceptible to acid, alkaline and neutral hydrolysis. The degradation products of thiocolchicoside and aceclofenac were well resolved from the pure drugs with significant differences in the retention time values. This method can be successfully employed for simultaneous quantitative analysis of thiocolchicoside and aceclofenac in bulk drugs and formulations.

A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of thiocolchicoside  and lornoxicam, using a RP-18 column and a mobile phase composed of 10mM ammonium acetate : methanol(50:50), pH7 adjusted with 1%triethyl amine. The retention time of thiocolchicoside and lornoxicam were found to be 4.6 and 10.2 min, respectively. Linearity was established for both thiocolchicoside and lornoxicam in the range of 1-10 µg/ml. The percentage recoveries of thiocolchicoside and lornoxicam were found to be 100.45±0.4489 and 100.70±0.5111, respectively. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, and photolytic degradation. The degradation studies indicated thiocolchicoside to be susceptible to acid, alkaline and neutral hydrolysis while lornoxicam showed degradation under acid and alkali. The degradation products of thiocolchicoside and lornoxicam were well resolved from the pure drugs with significant differences in the retention time values. This method can be successfully employed for simultaneous quantitative analysis of thiocolchicoside and lornoxicam in bulk drugs and formulations.

  Rapid and accurate isocratic reverse phase high performance liquid chromatography method is described for simultaneous determination of aceclofenac and thiocolchicoside in the combination dosage form. The separation of two drugs was achieved on Thermo Hypersil BDS C18 (250 mm X 4.6 mm) column of 5 µm particle size. Mobile phase consisted of 42:58 of acetonitrile and buffer of pH 6 respectively. Detection was carried out at 261 nm. Thermo Hypersil BDS C18 column showed most favorable chromatographic parameters for analysis. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution. The linear range for aceclofenac and Thiocolchicoside was 25-125 μg/ml and 1-6 μg/ml respectively.

A simple, economic and precise RP-HPLC method has been developed and validated for simultaneous estimation of thiocolchicoside (THC) and diclofenac (DCF) both in bulk drug and in capsule formulation. Reversed-phase chromatography was performed on a C18 Phenomenex-Gemini column with mobile phase acetonitrile: water (70:30 % v/v, adjusted at pH 3.0) at a flow rate of 1.0 ml/min. Detection was performed at 258 nm and sharp peaks were obtained for THC and DCF at a retention time of 1.537 min and 4.010 min respectively. The method was validated for accuracy, precision, detection and quantification limits, and system suitability in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed there was a linear relationship between response and concentration for THC in the range 4 - 24 μg/ml with the correlation coefficient 0.9998 and the linear regression equation y = 20.64x + 26.08. Linearity was observed in the range of 25 - 150 μg/ml with the correlation coefficient 0.9998 and the linear regression equation y = 11.13x + 65.27 for DCF. The detection (LOD) and quantification (LOQ) limits for THC were found to be 1.052 and 3.187 μg/ml and for DCF it was found to be 1.475 and 4.470 μg/ml. Statistical analysis proved that the method was precise, reproducible and accurate for simultaneous estimation of THC and DCF. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply that the method is suitable for routine quantification of THC and DCF with high precision and accuracy.