Ketoprofen

A new validated RP – HPLC method was developed for the simultaneous determination of Thiocolchicoside and Ketoprofen in combined dosage form. The method developed produced high sensitivity, precision and accuracy. An isocratic C18 (Inertsil ODS, 250 x 4.6 mm, 5µ) column was used with mobile phase of composition Acetonitrile: Phosphate buffer (70: 30 at pH 4.6) at a flow rate of 1.0 mL/min with UV detection at 258.2 nm for separating Thiocolchicoside and Ketoprofen. The retention time of Thiocolchicoside and Ketoprofen were 2.4 min and 3.5 min respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness as per ICH guidelines. Linearity for Thiocolchicoside and Ketoprofen were found in the range of 2.0 – 12.0 µg/ml and 6.2-38.75 µg/ml, respectively. The percentage recoveries for Thiocolchicoside and Ketoprofen ranged from 99.35- 100.21% and 98.66-99.29 %, respectively. The proposed method could be used for routine analysis of Thiocolchicoside and Ketoprofen in their combined dosage forms. All the proposed methods for Thiocolchicoside and ketoprofenare simple, selective, reproducible and specific with good precision and accuracy. The method was proved to be superior to most of the reported methods. These proposed methods for estimation of selected drugs were successfully applied either in tablet dosage form. More over the low solvent consumption along with short retention time of 2.4 and 3.5 for both Thiocolchicoside and Ketoprofen to be cost effective when compared to other developed method shown in literature reviews. The proposed method can be used as alternative methods to the reported ones for the routine determination of selected drugs under the study in tablet dosage form

One of the most feasible approaches for achieving a prolonged and predictable drug delivery profiles in the GIT is to control the gastric residence time (GRT) using gastroretentive dosage forms. The aim of the present study is to prepare the floating microspheres of ketoprofen to sustain the drug release for longer time to overcome the short half life of the drug. The microspheres were prepared by emulsification-solvent evaporation technique using ethyl cellulose and heat denaturation technique using egg albumin as a natural polymer. The optimization of microspheres was carried out based on pentagonal design using response surface methodology. The floating microspheres were evaluated for micromeritic properties, particle size, percentage yield, in-vitro buoyancy, entrapment efficiency, drug polymer compatibility, scanning electron microscopy and in-vitro drug release studies. The prepared microspheres exhibited prolonged drug release (> 9 h) and remained buoyant for > 24 h. The mean particle size increased and the drug release rate decreased at higher polymer concentration. The optimized formulation of ethyl cellulose microspheres (KEC-OP) exhibited prolonged drug release of 88.31 % up to 10 h demonstrating zero order kinetics and Case II transport release mechanism where as optimized formulation of egg albumin (KEA-OP) showed drug release of 96.78 % up to 9 h demonstrating peppas kinetics and Case II transport release mechanism.