reducing power

To establish a comparative account within the taxa by assessing its anti-oxidative property and mapping it with the morphometric characters. The methanolic leaf extracts of 12 Cassia L. species were screened for their antioxidant activity using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay and reducing power capability with reference to standard Ascorbic acid. Chamaecrista kleinii exhibited strong antioxidant activity with IC50 2.17 µg mL-1, followed with Senna auriculata (IC50 11.51 µg mL-1) and Senna polyphylla (15.17 µg mL-1). Highest reducing ability was observed in Senna auriculata extract with 0.676 nm absorbance. The correlation observed between the reducing power and DPPH radical scavenging assay supports the contribution of the phytoconstitutents like phenolics and flavonoids towards managing oxidative stress. The present study reveals the beneficiary effects of the selected plants by virtue of their antioxidant activity that can be harnessed in drug formulations. Keywords: Antioxidant, 1, 1-diphenyl-2-picryl hydrazyl, reducing power, Cassia.

Plant polyphenols are gaining increasing attention due to their potent antioxidant properties and marked effects in the prevention of various oxidative stress associated diseases. Identification and extraction of phenolic compounds from different plants has become a major area of health and medical research. In the present study, antioxidant activity of methanolic extracts of vegetative and reproductive parts of Moringa oleifera Lam. were tested for its free radical scavenging activity by spectrophotometric methods. Total phenolic and total flavonoid content were assayed spectrophotometrically. We observed high amount of total phenolic and flavonoid content in leaves and flowers of Moringa oleifera and this may be responsible for increased antioxidant activity in the plant. Antioxidant enzymes like ascorbate and ascorbate peroxidase (APX) were also found to be higher in vegetative and reproductive parts of Moringa oleifera. Our study reveals that M. oleifera leaves and flowers are potent source of natural antioxidants and the plant is valuable in pharmaceutical industry.

To evaluate phytochemical constituents and antioxidant potential of methanol aerial parts (leaf and stem) extract of Mentha arvensis L from Kashmiri Himalaya region. The extract shows the presence of flavonoids, saponins, alkaloids, steroids, carbohydrates, proteins, tannins and phenolics. The antioxidant activity of methanol aerial extract of Mentha arvensis L was evaluated by using 1,1-diphenyl, 2-picrylhydrazyl (DPPH) scavenging and reducing power assays. The total phenolic and total flavonoid content (mg/g) was found to be 672.11 and 283.1 respectively. The percentage inhibition of DPPH scavenging was found to be 81.33% at concentration of 0.5mg/ml. The reducing power was found to be 0.76 at 0.1mg/ml and increased to 2.42 at 0.5mg/ml. The results obtained revealed that methanol extract from aerial parts of Mentha arvensis L can be promising candidates for natural sources of antioxidants with high activity. 

This investigation is made upon the plant Artocarpus chaplasha, the bark of it, to find out its anti-oxidant property. The anti-oxidant property of this plant part was investigated using different solvent for extraction, such as ethanol, ethyl acetate, methanol and chloroform. Different types extract is used to see different extent of result as many phytochemicals are readily extracted in ethanol but not in methanol, and so on. In case of anti-oxidant the scavenging power (IC50) of DPPH radical was 55.15, 163.10, 166.94 and 407.875µg/ml for aforementioned extracts respectively. By the time IC50 ascorbic acid has 47.19µg/ml. There’s also test for total phenolic and flavonoid content was carried out and the ressult for each was 3.63, 61.97, 2.15 and 3.18mg/gm for each extracts respectively in terms of phenolic content. Total flavonoid content was 116.17, 121.51, 82.33 and 63.67mg/gm for each different extracts respectively.