isolation

The present research work is mainly concerned with the natural compounds that are obtained from traditional medicinal plants. There are number of natural compounds have been available in the nature. Some natural compounds are very useful to human being and have life potential to save human from many uncurable disease. Keeping view in the mind the research is focused to extract and isolate antioxidant, anti-inflammatory, anticancer, antihyperlipidemic, antidiabetic, antiulcer activities containing compounds are studied. The major natural compounds is ginger,   The ginger is obtained from rhizomes of zingiber officinale, and useful anti rhuematic and pungent and spicy contains gingerol and shogaol as active principles. All components are isolated from the concerned extracts. The extraction   of all plants are based on successive solvent extraction method for all drugs.The constituents are confirmed by structure elucidation. The structure of each compounds are intrepreted by different spectral techniques like Infrared spectrum, nuclear magnetic spectrum (hydrogen anc carbon thirteen spectra) and mass spectroscopy for molecular  formula and molecular weight of the unknown compounds.  The biological evaluation of each compound is performed separately. For antioxidant activity DPPH method is used. & the immunomodulatory activity

Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads. Due to an increasing demand for seeking therapeutic drugs from natural products there is a need to develop analytical methodologies for different purposes which include the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations. Various analytical methods available for the isolation, characterization and quantification of active ingredients from plant extracts which include various spectrometric and chromatographic methods along with some hyphenated techniques are discussed. The present review helps to design a method for the isolation, characterization and quantitative determination of major constituents from plants.