Thin layer chromatography

The aim of this work is to establish simple economical, and rapid spectrophotometric and chromatographic identification method for qualification of captopril in Active Pharmaceutical Ingredient. The work was carried out to for estimation of captopril in bulk pharmaceutical from by utilizing area under curve (AUC)method using UV –Visible Spectrophotometry .For this purpose the wavelength range 200-400 nm was selected. Thin-Layer chromatography (TLC) is a chromatography used to separate non-volatile mixtures. TLC can be used for monitoring the progress of a reaction ,identification compounds present in a given mixture, and determination of active pharmaceutical ingredient .TLC analysis of captopril in pharmaceutical and for using this method of analysis in future for the development of bio analytical method .for TLC the mobile phase with different concentration was used .we have established that the most perfect RF observed using mobile phase .chloroform –methanol are used (9:1)and (8:2) ratio .the development method was found to be simple ,linear ,precise ,accurate and sensitive which can be used for routine analysis for spectrophotometric and Thin-layer chromatographic method estimation of active pharmaceutical Ingredient.

Standardization of medicinal plants is an important step as it determines the identity and quality of a plant. The aim of present study was to standardize Polygonum glabrum by evaluating physicochemical parameters. Successive extraction, phytochemical screening and thin layer chromatography analysis were studied to detect the presence of various phytoconstituents. Physicochemical parameters such as loss on drying, total ash, acid insoluble ash, water soluble extractive and alcohol soluble extractive values were found to be 06.7±1.19%w/w, 15.52±0.28%w/w, 4.65±0.02%w/w, 20.05±1.07%w/w and 13.66±0.20%w/w, respectively. In phytochemical screening, petroleum ether extract revealed the presence of steroids, tannins and saponins whereas chloroform extract confirmed the presence of alkaloids, flavonoids and carbohydrates. Ethylacetate extract showed the presence of phenolic compounds, steroids, proteins and glycosides. Further, major constituents like alkaloids, sugars, phenols, proteins, steroids, saponins, tannins and flavonoids were present in methanol extract. In addition, thin layer chromatography analysis supports the presence of terpenoids, alkaloids and flavonoids in petroleum ether, chloroform and methanol extracts, respectively.  It can be concluded that the standardization parameters investigated in this study could assist in safe, accurately collection, handling of crude materials, ensure efficacy and stability of ended product. Further, the major active constituents were identified in different extracts of Polygonum glabrum

Two sensitive and reproducible methods are described for the quantitative determination of zonisamide in the presence of its degradation products. The first method was based on high performance thin layer chromatographic (HPTLC) followed by densiometric measurements of their spots at 238 nm.the separation was on HPTLC aluminium sheets of silica gel 60 F254, using toluene:ethtyl acetate (6:4 v/v), this system was found to give compact spots for zonisamide after development (Rf value 0.49.). The second method based on high performance liquid chromatographic (HPLC) of the drug from its degradation products on reversed phase Vydac column ODS (250 mm × 4.6 mm, 5.0 µ), at ambient temperature using a mobile phase consisting of methanol: water (50:50, v/v) and Retention time was (4.71 min.) Both the methods were validated as ICH guidelines no chromatographic interference from capsule excipients was found. Zonisamide was subjected to acid and alkali hydrolysis, oxidation,and dry heat. The drug was fount to be stable in neutral wet heat and photo- degradation conditions as the proposed analytical methods could effectively separated the drug from its degradation products, they can be employed as stability-indicating.

  The Present paper deals with pharmacological aspects and phytochemical screening of G. superba Linn. by Thin layer chromatography. The extract showed the presence of various phytocompounds like alkaloids and flavonoids. TLC profiling of G. superba linn extract give an idea about the presence of various phytochemicals. Different Rf (Retention factor) value of various phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals. In vitro antioxidant activity was carried out with the extract using Nitric oxide assay with ascorbic acid as a standard. The extract had shown significant antioxidant activity. The results of the Nitric oxide scavenging activity study indicate a concentration dependent antioxidant activity. The total phenolic and flavonoid content was found to be 22.3 ± 3 µg g-1Gallic acid equivalents (GAE) and the flavonoid was 47 ± 5.4 µg g-1 quercetin equivalents. The Nitric oxide scavenging activity of the extract was found to be promising and IC50 of extract was 119.06±.19. It indicates that the hydro alcoholic leave extract of the plant has the potency of scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from Indian medicinal plants to be used in treating diseases related to free radical reactions. Key words: Thin layer chromatography, Gloriosa superba linn., Antioxidant activity.