AUC

The aim of this work is to establish simple economical, and rapid spectrophotometric and chromatographic identification method for qualification of captopril in Active Pharmaceutical Ingredient. The work was carried out to for estimation of captopril in bulk pharmaceutical from by utilizing area under curve (AUC)method using UV –Visible Spectrophotometry .For this purpose the wavelength range 200-400 nm was selected. Thin-Layer chromatography (TLC) is a chromatography used to separate non-volatile mixtures. TLC can be used for monitoring the progress of a reaction ,identification compounds present in a given mixture, and determination of active pharmaceutical ingredient .TLC analysis of captopril in pharmaceutical and for using this method of analysis in future for the development of bio analytical method .for TLC the mobile phase with different concentration was used .we have established that the most perfect RF observed using mobile phase .chloroform –methanol are used (9:1)and (8:2) ratio .the development method was found to be simple ,linear ,precise ,accurate and sensitive which can be used for routine analysis for spectrophotometric and Thin-layer chromatographic method estimation of active pharmaceutical Ingredient.

A Quantitative High Performance Thin Layer Chromatography (QHPTLC) method was developed for the estimation of ‘Candana’ (Santalum album), one of the herbal ingredients present in the Ayurvedic polyherbal formulation ‘Padoladi Ghritham’. For this, both the methanol extracts of ‘Candana’ crude drug and Padoladi Ghritham were run using a mobile phase Chloroform : Isopropyl alcohol : Toluene (8:1:1, v/v/v) on a silica G 60 F254 stationary phase. Methanol extract of Candana  gave a spot with Rf value of 0.76. The methanol extract of Padoladi Ghritham also gave a spot with Rf value of 0.76. The chemical equivalency of these two spots was revealed by the same pattern of the UV spectrum with a λmax of 290 nm. Then the estimation was done graphically by preparing Quarter Standard, Half Standard, Normal Standard, and Double Standard of the formulation. A calibration plot was drawn between the concentration of standards and peak area of the reference spots. The concentration of Candana in the samples was found from the corresponding AUC obtained for the reference peak of the samples. A regression coefficient of 0.989 was obtained. The developed QHPTLC method is a simple and accurate one and can be used as a tool for the quantification of each ingredient in a polyherbal formulation.

Hydrotropicsolvents may proper choice to preclude the use of organic solvents so that, a simple, accurate, novel, safe and precise method could developed for estimation of poorly water soluble drug,. Solubility of Daurnavirincreased by using 8M urea as a hydrotropic agent. Daurnavir1showed the maximum absorbance at 263.91nm in method A,254-274 nm in method B and 264nm in method C. At these wavelengths, hydrotropic agent and other tablet excipients did not show any significant interference in the spectrophotometric assay. The developed methods were found to be linear in the range of5-40 µg/ml for method A, method B&C with correlation coefficients (R) of 0.997, 0.995 and 0.997 respectively.The mean percent label claim of tablets of of Daurnavir in formulation estimated by the proposed methods was found to be107%. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical parameters were found to be good accordance with the prescribe values. As hydrotropic agent was used in the proposed methods, these methods were eco-friendly and it can be used in routine quantitative analysis of drug in bulk and dosage form in industries. Key words:Daurnavir, urea;  AUC; Hydrotropicsolubilization technique; derivative spectroscopy.

Hydrotropicsolvents may proper choice to preclude the use of organic solvents so that, a simple, accurate, novel, safe and precise method could developed for estimation of poorly water soluble drug,. Solubility of ofloxacin is increased by using 8M urea as a hydrotropic agent. Ofloxacin showed the maximum absorbance at 288 nm in method A, 284-292 nm in method B and 316nm in method C. At these wavelengths, hydrotropic agent and other tablet excipients did not show any significant interference in the spectrophotometric assay. The developed methods were found to be linear in the range of 3-15 µg/ml for method A, 1.5-7.5 µg/ml for method B&C with correlation coefficients (R) of 0.999, 0.996 and 0.992 respectively. The mean percent label claim of tablets of ofloxacin in formulation estimated by the proposed methods was found to be97.52%. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical parameters were found to be good accordance with the prescribe values. As hydrotropic agent was used in the proposed methods, these methods were eco-friendly and it can be used in routine quantitative analysis of drug in bulk and dosage form in industries.