Streptozotocin

The anti-diabetic effect of aqueous and ethanol extract of leaves of Eugenia operculata Roxb. was evaluated in streptozotocin induced diabetic mice. Streptozotocin is a synthetic nitrosoureido glucopyranose derivative isolated from fermentations of Streptomyces acromogenes. STZ induced diabetes by selectively destroying the insulin producing β cells by inducing necrosis. Extract (250mg/kg b.w) was administered orally to albino mice both normal and diabetic for 21 days. The anti-diabetic potential was assessed by determining oral glucose tolerance, fasting blood glucose, urine glucose, change in body weight, liver glycogen content and serum lipid profile. In oral glucose tolerance test, reduction of fasting blood glucose level was observed after 60 minutes of extract and standard administration. At the end of experiment maximum reduction of blood glucose level was seen significantly (p

The chronic type-2 diabetes mellitus leads to diabetic nephropathy, which is one of the major microvascular complication of end stage renal disease worldwide and causes premature death in diabetic patients. The objective of the present investigation was to evaluate the antidiabetic activity and protective effect on diabetic induced nephropathy of combinations of ethanolic root extract of Asparagus racemosus (REAR) and seed extract of Eugenia jambolana(SEEJ) by using in-vivo model. The in-vivo antidiabetic activity and the effect on diabetic nephropathy was evaluated on streptozotocin- nicotinamide induced type-2 diabetes mellitus in male albino Wistar rats. The in-vivo study showed that blood glucose level was significantly reduced in dose dependent manner when compared to the diabetic control group. In addition, it significantly restored the body weight loss, increased kidney weight, glycosylated haemoglobin, blood urea, blood uric acid, blood urea nitrogen, blood creatinine, urine volume and urine microalbumin levels when compared to diabetic control groups. The report of histopathological study of rat kidney tissues strongly supported the protective effect of combinations of REAR and SEEJ in diabetic nephropathy. The findings of this investigation concluded that combinations of REAR and SEEJ has significant antidiabetic activity and potential protective effect in diabetic nephropathy.