PCR

HLA-B27 test is generally positive in spondyloarthritis (SpA) and ankylosing spondylitis (AS). Several anomalous laboratory test results are frequently found in HLA-B27-positive patients. In this study, we intended to evaluate two groups of HLA-B27 positive patients- one group belonging to the 13-40 years age group and another group belonging to the 41-71 years age group. The rationale of this partition was based on the age when AS first emerged and the age when the disease was set up for quite a few years respectively. We anticipate alterations of several familiar laboratory test outcomes between these two groups. After our analysis, we found that in the upper age group, ESR and uric acid are significantly decreased, while SGPT, creatinine, and HbA1C are significantly increased. Other values like ct value in PCR, RA seropositivity, and TC of WBC are decreased a bit in the higher age group; haemoglobin and CRP also marginally increased in this set which are not statistically significant. The plausible explanations behind these changes are discussed.

This presented work is based on application of two multivariate calibration methods for simultaneous UV-Visible spectrophotometric determination of active substances in combined pharmaceutical formulation contained of Tinidazole (TINI) and Norfloxacin (NFX). The methods used were Partial Least Square (PLS) and Principal Component Regression (PCR). The spectra of both NFX and TINI were recorded at concentrations within their linear range 2.0-12.0 μg/mL for NFX and 5.0-30.0 μg/mL for TINI. The 29 set of mixtures were used for calibration and 07 set of mixtures were used for validation in the wavelength range of 260 to 320 nm with the wavelength interval λ= 0.2 nm in methanol. The methods were validated as per International Conference on Harmonization Q2 (R1) (ICH) guidelines. These methods were successfully applied for determination of drugs in pharmaceutical formulation (tablet) with no interference of the excipients as indicated by the recovery study results. The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control as well as in process control of drugs and formulation.

This study aims to study the diversity of bacteria in different water samples which taken from Meet Faris drinking water station, during the period from July, 2013 till June, 2014. The bacteriological analyses involved total bacterial counts (TBCs), total coliforms (TC) and estimation of fecal coliforms (FC), fecal streptococci and pathogenic bacteria. Fifty pathogenic bacterial isolates obtained from raw water samples. The most prevalent organisms were E.coli (30%), Klebsiella.spp (14%), Pseudomonasspp(16%), Proteus. Spp (10%), E. fecalis (14%) Shigellaspp.(8%), Staph.coccus (8%). According to antibiotic susceptibility test results, enrofloxacin and gentamicin were the most effective antibiotic on E.coli isolates. The obtained results revealed that, the River Nile water was subjected to sewage pollution. Detection of E.coli by PCR was found to be important for confirmation of the detected E.coli isolates.