Moxifloxacin

Two simple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of difluprednate and moxifloxacin hydrochloride in combined dosage form have been developed. First method employs formation and solving of simultaneous equation using 241nm and 288 nm as two analytical wavelengths for both drugs in distilled water. The second method is a Q value analysis based on measurement of absorptivity at 241nm and 264nm (as an ISO-absorptive point). Difluprednate at their respective λmax 241 nm and 288 nm and at iso-absorptive point 264 nm shows linearity in a concentration range of 10-20 μg/mL and similarly moxifloxacin at their respective λmax 288 nm and 241 nm and at iso-absorptive point 264 nm shows linearity in a concentration range of 1-5 μg/mL. The results of the analysis have been validated statistically.

A simple and economical Q-Absorbance spectrophotometric method has been developed for the simultaneous estimation of cefixime and moxifloxacin in their synthetic mixture. The method is based on Q‐absorption Ratio method using two wavelengths, 293.6 nm (λmax of Moxifloxacin) and 276 nm (Isoabsorptive point). Methanol was used as a solvent in the method. Both drugs showed linearity in the range of 4‐14 μg/mL in the methods. The method was validated statistically and recovery studies were carried out. The proposed method was found to be simple, economical, accurate, and reproducible. It can be applied for routine analysis and quality control of both drugs in their combination drug products.

A reaction between Moxifloxacin HCl and FeCl3 solution in slightly acidic medium was studied. It gave brown colour complex having maximum absorbance at 407.0nm. The reaction is selective for Moxifloxacin HCl with 0.00024µg/uL and different concentration of FeCl3 was applied.  The color reaction obeyed Beer’s law from 0.01 µg /µL to 0.00018 µg/µL of FeCl3 solution having concentration 0.1ug∕uL and relative standard deviation 0.026%.The quantitative estimation of moxifloxacin HCl in blood sample is also studied. Key Words: Moxifloxacin, UV-Visible Spectrophotometer, Ferric Chloride, Beer’s law, Color reaction, Analytical method