UV spectroscopy

The study describes the simple, sensitive, accurate, rapid and reliable ultra violet spectroscopic method has been developed for determination of Deferiprone in bulk drug and pharmaceutical formulation. Deferiprone is use as second line agent for thalassemia when iron overload from blood transfusion occurs.in order to investigate the stability of drug, a stress testing of drug sample by exposing it to variety of force degradation conditions has been recommended. Deferiprone was subjected to stress degradation under different condition recommended by international conference on harmonization (ICH). Deferiprone shows maximum absorbance at 279nm & calibration graph linear in the concentration range 5-25 Mcg/ml with correlation co-efficient 0.9997.The higher percentage of recovery study indicates that there is no interference of excipients in the presence of formulation. The stability study indicates appreciable changes were observed by treating the drug with acidic hydrolysis, basic hydrolysis and oxidation. However, there is no appreciable changes were observed for thermal stress and photolytic degradation.

Two simple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of difluprednate and moxifloxacin hydrochloride in combined dosage form have been developed. First method employs formation and solving of simultaneous equation using 241nm and 288 nm as two analytical wavelengths for both drugs in distilled water. The second method is a Q value analysis based on measurement of absorptivity at 241nm and 264nm (as an ISO-absorptive point). Difluprednate at their respective λmax 241 nm and 288 nm and at iso-absorptive point 264 nm shows linearity in a concentration range of 10-20 μg/mL and similarly moxifloxacin at their respective λmax 288 nm and 241 nm and at iso-absorptive point 264 nm shows linearity in a concentration range of 1-5 μg/mL. The results of the analysis have been validated statistically.