Beer’s law

The main aim of current study was to develop U V visible spectrophotometric method for the quantitative determination of Flucloxacillin and  Ceftriaxone in bulk and  pharmaceutical formulations. Flucloxacillin  is a  broad spectrum beta -lactam antibiotic, belonging to the isoxazolyl family of penicillin’s. Ceftriaxone is a semi synthetic, broad-spectrum and third generation  cephalosporin antibiotic for intravenous or intramuscular administration. The solvents used in this method is chloroform ,double distilled water (1:1) in presence of phosphate buffer of pH 7.4. Linearity test solutions for the assay method were prepared in the range of 2-12 μg/ mL and  4-16 μg/mL respectively for determination of flucloxacillin and ceftriaxone which obeyed Beer’s law and found to linear. From the experimental results reveal that absorption maximum of 270 nm for flucloxacillin and 290 nm for ceftriaxone were found. Validation of the developed method for two drugs was carried out as per ICH requirements.

A reaction between Moxifloxacin HCl and FeCl3 solution in slightly acidic medium was studied. It gave brown colour complex having maximum absorbance at 407.0nm. The reaction is selective for Moxifloxacin HCl with 0.00024µg/uL and different concentration of FeCl3 was applied.  The color reaction obeyed Beer’s law from 0.01 µg /µL to 0.00018 µg/µL of FeCl3 solution having concentration 0.1ug∕uL and relative standard deviation 0.026%.The quantitative estimation of moxifloxacin HCl in blood sample is also studied. Key Words: Moxifloxacin, UV-Visible Spectrophotometer, Ferric Chloride, Beer’s law, Color reaction, Analytical method