Methanolic extract

The global rise in antimicrobial resistance (AMR) has intensified the search for plant-derived alternatives with therapeutic potential. Murraya koenigii (L.) Spreng., a plant valued in traditional medicine, is rich in bioactive compounds, though its bark has not been extensively studied. This research aimed to analyze the phytochemical constituents and evaluate the antibacterial properties of the methanolic extract of M. koenigii bark sourced from the Botanical Garden of VJ’s College of Pharmacy, Rajahmundry. The bark was dried, ground, and extracted using methanol via maceration. Standard qualitative tests were used to identify secondary metabolites such as alkaloids, flavonoids, tannins, saponins, triterpenoids, and cardiac glycosides. The extract’s antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using the agar well diffusion method, with Amikacin as the positive control and methanol as the negative control. The extract demonstrated a rich phytochemical profile and showed dose-dependent antibacterial effects. Maximum inhibition was observed at 400 mg/mL with zones of 15.3 mm (S. aureus), 13.8 mm (E. coli), and 12.5 mm (P. aeruginosa). The study indicates that M. koenigii bark methanolic extract possesses significant antibacterial activity, suggesting its potential as a plant-based antimicrobial agent.

Terminalia pallida brandis and Boswellia ovalifoliolata most commonly found plants in the south India. Methanolic and nHexane extract of Terminalia pallida roots and Boswellia ovalifoliolata roots were analysed for antioxidant activity. The In-vitro methods for antioxidant activity are based on the principle of inhibition of free radicals.Present study provides focus on the reducing power assay and the free radical scavenging activity of both the plants in both the extracts. It was observed that Terminalia pallida roots and Boswellia ovalifoliolata roots have demonstrated dose dependent reducing property.

  The present research has been undertaken with the aim to formulate and evaluate the herbal gel containing Calophyllum inophyllum extract. The gel formulation was designed by using methanol extract of Calophyllum inophyllum stem barks in concentration (5%) and evaluated using physiological measurements. The gel was prepared by using accurately weighted amount of drug along with other additives were poured into the fixed amount of hydrated Carbopol-934 dispersion with constant stirring. Finally the required amount of 0.5M sodium hydroxide solution was added to induce gelation. All the prepared gel formulations were subjected for preliminary evaluation such as pH, Viscosity and Rheological studies, Spreadability, Drug content uniformity, Skin irritation test, In vitro diffusion study, In vitro permeation studies, Drug Polymer Compatibility Studies. The optimized herbal gel formulation of the drug was subjected to accelerated stability studies at both 40C and 370C for about 3 months. A suitable UV method was developed for herbal gel formulation by using Phosphate buffer 6.8 as solvent and lmax found to be 284 nm. The pH of all the formulations was in the range of 6.63 to 7.35, which lies in the normal pH range of the skin. The drug content was in the range of 96.6 to 99.5 %. The formulations did not produce any skin irritation, i.e., erythema and edema for about a week, when applied over the skin. The drug interaction FT-IR studies indicated that there was no chemical interaction between the drug and the polymers used in gel formulations.   Key Words: Calophyllum inophyllum, Methanolic extract, Herbal gel formulations, Carbopol 934, pH, Phosphate buffer.