Levosulpiride

A simple, precise and accurate RP-HPLC method was developed for the separation and quantification of  Ilaprazole and Levosulpiride in pharmaceutical dosage form. The quantification was carried out using Hypersil BDS C8 column ( 150x 4.6mm,5µm) and mobile phase comprised of methanol and phosphate buffer(pH 7.5 adjusted with 0.01 M NaOH) in proportion of ratio 70:30 degassed under ultra- sonication. The flow rate was adjusted to 1 ml/min and the effluent was monitored at 242nm using PDA detector. The method was validated in terms of linearity, precision, accuracy and specificity, limit of detection and limit of quantization. Linearity of Ilaprazole and Levosulpiride were in range of 10-60 µg/ml and 75-450 µg/ml respectively. The retention time of Ilaprazole and Levosulpiride were 6.52 and 2.02 respectively. The percentage recoveries of both drugs were found to be 100.03%  and 100.18% for Ilaprazole and Levosulpiride respectively from the capsule formulation. The method was found to be precise, accurate and specific during the study. The proposed method enables rapid quantification and simultaneous analysis of both drugs from commercial formulations without any excipients interference. The method can be used for routine analysis of marketed product of Ilaprazole and Levosulpiride in combined capsule formulation.

A simple, precise, accurate, rapid and economical spectrophotometric method have been developed for simultaneous estimation of Pantoprazole sodium and Levosulpiride in pure and in combined capsule dosage form. Method-1 simultaneous equations and Method-2 Q-absorbance Ratio method by using 287 nm and 231 nm as absorbance maxima (λ max)for Pantoprazole  sodium and Levosulpiride  respectively and 248 nm (isoabsorptive point). A methanol was used as Solvent. Linearity was observed in the concentration range of 5-30 µg/ml for Pantoprazole sodium and 5-30 µg/ml for Levosulpiride. The method was validated statistically and recovery study was performed to confirm the accuracy of the method. 

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic method for the simultaneous determination of Pantoprazole and Levosulpiride in pharmaceutical dosage form. In this study a first-derivative spectroscopic method was used for simultaneous determination of pantoprazole and levosulpiride using the zero-crossing technique. The measurements were carried out at wavelengths of 269 and 249 nm for Pantoprazole and Levosulpiride respectively. The method was found to be linear (r2>0.9929) in the range of 10-50 μg/ml for Pantoprazole at 269 (ZCP of Levosulpiride) nm. The linear correlation was obtained (r2>0.9948) in the range of 10-50 μg/ml for Levosulpiride at 249 (ZCP of Pantoprazole) nm. The limit of determination was 0.69 and 0.58 μg/ml for pantoprazole and levosulpiride respectively. The limit of quantification was 2.06 and 1.69 μg/ml. The method was successfully applied for simultaneous determination of Pantoprazole and Levosulpiride in pharmaceutical dosage form.