UV Spectrophotometric

A simple, precise, accurate, rapid and economical spectrophotometric method have been developed for simultaneous estimation of Pantoprazole sodium and Levosulpiride in pure and in combined capsule dosage form. Method-1 simultaneous equations and Method-2 Q-absorbance Ratio method by using 287 nm and 231 nm as absorbance maxima (λ max)for Pantoprazole  sodium and Levosulpiride  respectively and 248 nm (isoabsorptive point). A methanol was used as Solvent. Linearity was observed in the concentration range of 5-30 µg/ml for Pantoprazole sodium and 5-30 µg/ml for Levosulpiride. The method was validated statistically and recovery study was performed to confirm the accuracy of the method. 

A simple, rapid, precise, and economical spectrophotometric method has been developed for quantitative analysis of Rilpivirine hydrochloride (RILH) in manufactured tablet formulations. The initial stock solution of RILH was prepared in dimethyl formamide: acetonitrile solvent and subsequent dilution were done in acetonitrile. The standard solution of RILH in acetonitrile showed absorption maxima at 281.6 nm. The drug obeyed Beer–Lambert’s law in the concentration range of 1–16 μg/mL with coefficient of correlation (R2) was 0.9999. It showed coefficient of variation below 2 % in intra-run and inter-run precision.  The results of analysis have been validated as per ICH guidelines. The method can be adopted in routine analysis of RILH in bulk and tablet dosage form and it involves relatively low cost solvents and no complex extraction techniques.