Acute toxicity

The concerned study reveals antidiabetic effects of different polyherbal combinations of two medicinal plants used in traditional medicine. Aim of the present study was to evaluate antidiabetic action of polyherbal combination of two medicinal plants. Methanol: water (70:30) extracts of Carica papaya and Curry leaf were used for polyherbal combinations. All these combinations were studied for their acute toxicity and 200 mg/kg dose was selected. OGTT, antidiabetic activity and liver function tests were performed for all the combinations. Reduction in blood glucose level was determined in antidiabetic activity for 0 to 20 days and histopathology of the pancreas was performed after 20th day. Results revealed that all combinations were safe and dose was selected at 200 mg/kg. Polyherbal combinations II showed significant antidiabetic activity in OGTT and STZ-diabetic rats. Treatment with combination-II in diabetic animals produced beneficial improvement in lipid profile. Histopathological observations showed improvement in the rat treated with combination-II. It may be concluded that combination-II was most effective and safe in comparison to other combinations. Flavonoids, tannins and sterols present in this combination might be responsible for the effect.