Hiren Kadikar

Accurate, precise, rapid and economical first order derivative spectroscopic method was developed and validated for the estimation of Cefixime and levofloxacin in tablet. The wavelengths selected for quantitation were 289.45 nm for levofloxacin (zero cross for cefixime) and 317.0 nm for cefixime (zero cross for levofloxacin). Linearity for detector response was observed in the concentration range of 2-12 μg/ml for both Cefixime and Levofloxacin using methanol as a solvent with correlation coefficient 0.991 and 0.993 respectively. The proposed method was successfully applied for the simultaneous estimation of both drugs in tablet.

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic method for the simultaneous determination of Pantoprazole and Levosulpiride in pharmaceutical dosage form. In this study a first-derivative spectroscopic method was used for simultaneous determination of pantoprazole and levosulpiride using the zero-crossing technique. The measurements were carried out at wavelengths of 269 and 249 nm for Pantoprazole and Levosulpiride respectively. The method was found to be linear (r2>0.9929) in the range of 10-50 μg/ml for Pantoprazole at 269 (ZCP of Levosulpiride) nm. The linear correlation was obtained (r2>0.9948) in the range of 10-50 μg/ml for Levosulpiride at 249 (ZCP of Pantoprazole) nm. The limit of determination was 0.69 and 0.58 μg/ml for pantoprazole and levosulpiride respectively. The limit of quantification was 2.06 and 1.69 μg/ml. The method was successfully applied for simultaneous determination of Pantoprazole and Levosulpiride in pharmaceutical dosage form.