Amir

In indigenous/traditional systems of medicine, the drugs are primarily dispensed as water decoction or ethanolic extract. Fresh plant parts, juice or crude powder are a rarity rather than a rule.  Total Phenolic content in the extracts measured as gallic equivalent in Ashwagandha (methanolic)  43.77 ± 1.7, aqueous = 42.52 ± 0.8, activity of Trolox by ABTS method IC50 value 428.38 ± 1.90 µg /ml, Total Antioxidant activity by ABTS method in Ashwagandha (methanolic) IC50 Value is 28.353 ± 0.269 mg/ml in  aqueous 32.547 ± 0.532 mg/ml , Free radical scavenging capacity by DPPH in Ashwagandha (methanolic) IC50 Value 2.9  ± 0.09 mg/ml  in aqueous 3.27 ± 0.05 mg/ml. The medicinal plant examined in this study has long been used in Indian traditional medicine, Ayurveda as well as many other traditional drug practices of the rest of the world. Withania somnifera, known as ‘Ashwagandha’, has been an important herb in the Ayurvedic and indigenous medical systems for centuries in India.

For the evaluation of the wound healing activity polymer composite films were prepared by using Chitosan and in combination with Sodium CMC with and without glutaraldehyde were prepared by solvent casting method. Mupirocin was incorporated into selected polymeric films. All the polymeric composite films were characterized by IR study suggested that there was no chemical reaction has taken place, only ionic complexes were formed. All the films were evaluated for thickness, folding endurance and tensile strength. The thickness of all the films was uniform,The folding endurance suggested good flexibility of the films as propylene glycol was used as a plasticizer, Films shown good tensile strength  necessary for better handling . The water vapour penetration suggested that films without cross linker absorbs more moisture compared to films containing cross linking agent. The presence of cross linking agent shown optimum bio-adhesion. All the polymer composite films were evaluated for in vitro swelling study. The films showed good swelling in water more than 6 hrs retaining the shape of the films. The addition of cross linking agent decreased the swelling. Selected polymer composite films were evaluated for in vivo wound healing activity. All the polymeric films showed more than 80% reduction in wound contraction. The mupirocin loaded polymeric composite containing Sodium CMC, shown more than 95% of reduction in wound area after 12 day. Hence it can be concluded that polymer composite films of chitosan-alginate containing mupirocin along with Sodium CMC showed good wound healing and could be used in effective management of wound.

The main anti-acne medicines including retinoids, systemic and topical antibiotics and hormones have several severe side effects. According to the literature, it is expected that Calendula officinalis, Rosa canina, Zataria multiflora and Trigonella foenum graecum which have antibacterial, anti-inflammatory and anti-oxidant effects, and Glycin max that have phytosterogenic property, heal the inflamed lesions of the disease. The purpose of the present study was to formulate and clinically evaluate an anti-acne cream using the extract of the above mentioned plants.A general formula of a cleansing cream containing bees wax, spermaceti, borax, liquid paraffin and water was considered and then corrected. The best formula was chosen according to its physicochemical properties. The stability of the formulation was evaluated. After diagnosis of acne vulgaris, the number of inflamed lesions and severity of the disease were determined. After two weeks of administration of the coded formulations, the process of improvement and possible complications were reviewed. If necessary, the treatment was continued for another two weeks. Finally, the patients were examined for the number of lesions and the severity again. The significance of data was analyzed statistically using SPSS. The final product was water in oil cream with suitable appearance and acceptable physico-chemical stability. Clinical trial results were significantly illustrated the effectiveness of the formulation mainly against papular and pustular lesions; disease severity and acne-induced inflammation, when compared to placebo. Previous researches have shown the presence of steroid saponins in Trigonella foenum graecum that make it a good anti-inflammatory agent. Also, anti-oxidant and anti-microbial effect of the other above mentioned plants are reported. Considering the results of the present study, when compared with placebo, a formulation containing the mixed extract of the plants has a good efficacy in the treatm