Alhat Vidya N

Biodiesel is a renewable, clean burning diesel replacement that is reducing dependence of foreign petroleum, creating jobs and improving the environment. Made from recycled cooking oil, soya bean oil and animal fats. Growing concern regarding energy resources and the environment has increased interest in the study of alternative sources of energy. To meet increasing energy requirements there has been growing interest in alternative fuel. The utilization of liquid fuels such as biodiesel produced from cooking oil by transesterification process. The most promising options for the use of conventional fossil fuels. 

The aim of this work is to establish simple economical, and rapid spectrophotometric and chromatographic identification method for qualification of captopril in Active Pharmaceutical Ingredient. The work was carried out to for estimation of captopril in bulk pharmaceutical from by utilizing area under curve (AUC)method using UV –Visible Spectrophotometry .For this purpose the wavelength range 200-400 nm was selected. Thin-Layer chromatography (TLC) is a chromatography used to separate non-volatile mixtures. TLC can be used for monitoring the progress of a reaction ,identification compounds present in a given mixture, and determination of active pharmaceutical ingredient .TLC analysis of captopril in pharmaceutical and for using this method of analysis in future for the development of bio analytical method .for TLC the mobile phase with different concentration was used .we have established that the most perfect RF observed using mobile phase .chloroform –methanol are used (9:1)and (8:2) ratio .the development method was found to be simple ,linear ,precise ,accurate and sensitive which can be used for routine analysis for spectrophotometric and Thin-layer chromatographic method estimation of active pharmaceutical Ingredient.