Zero order derivative spectroscopy

Effective and advantageous Hydrotropic Solubilization technique has been developed for the estimation of two drugs i.e, Flurbiprofen and Glipizide in bulk and its pharmaceutical formulations. Hydrotropic Solubilization technique is one of the aqueous solubility enhancing methods employed for the poorly water soluble drugs and found to be simple, precise and cost effective. Solvents like Piperazine, Urea, Sodium Salicylate, Sodium benzoate etc are the commonly used as hydrotropic solventsin different concentrations. The use of these hydrotropic solvents are of proper choice since the use of organic solvents can be reduced as it is hazardous, costlier and causes environmental pollution. In this context, 1M piperazine has been used as a solubilizing agent to enhance solubility of both the drugs, Flurbiprofen and Glipizide. The absorption maximum of Flurbiprofen and Glipizide was found to be at 246nm and276nm in Zero order derivative spectrum (Method A), calculation of Area Under Curve (AUC)(Method B) was done in the wavelength range of 236-256nm for Flurbiprofen and 266-286nm for Glipizide. The Beer-Lambert’s law has been followed in the concentration range of 2-10µg/ml for Flurbiprofen and 5-35µg/ml for Glipizide for both the methods. The methods were validated as per ICH guidelines and all the validation parameters were found to be within the acceptable range. The developed methods were successfully practiced to estimate the amount of Flurbiprofen and Glipizide in bulk and pharmaceutical dosage forms in routine analysis.