Triphala

The importance of mouth and teeth cleanliness has been recognized from the earliest days of civilization to the 21st century. Patients and oral health practitioners are faced with a multitude of mouthwash products containing many different active and inactive ingredients. Making informed decisions as to the suitability of a particular product for a particular patient can be a complex task. Although many popular herbal products have helped to control dental plaque and gingivitis, they have been used for a short time and only as an adjunct to other oral hygiene measures such as brushing and flossing. Various herbal products and their extracts such as Turmeric, Triphala, Neem, Tulsi, etc, have shown significant advantages over the chemical ones. Natural mouthwashes may offer significant advantages over the chemical ones. If such mouthwashes can be formulated which can be easily prepared and used safely by people at home using natural products, it may lead to improvement in the general dental health of the population. We have prepared formulation F1 to F5. Then according to antimicrobial activity and evaluation parameters optimized one formulation.

The current investigation was delineated to find out the anticancer activity of three different formulations of Triphala and to find the best Triphala formulation for anticancer activity among the three. Aqueous extracts of Terminalia chebula, Termialia bellerica and  Phyllanthus emblica were obtained from Amruta herbals, Indore was dissolved in purified water to make Triphala 1:1:1, 1:2:3, 1:2:4 (F1, F2, F3 respectively) formulation. The number of 1x106 Ehrlich ascites carcinoma (EAC) cell was inoculated in mice. Group I served as untreated negative control while group II was considered as positive control. Group III cancerous animals received standard drug cyclophosphamide 25 mg/kg b.w., whereas group IV to VI cancerous animals were treated with F1 150, 300 and 600mg/kg b.w., group VIII to IX with F2 150, 300 and 600 mg/kg b.w and group X to XII with F3 150, 300 and 600mg/kg b.w., respectively. Different parameter such as change in body weight, determination of survival time, total ascites fluid volume, packed cell volume and haematological parameters were perceived. At the end of investigation, 6 animals from each group were immolated, blood samples were collected and WBC, RBC, Hb content was reckoned. The effect of all three formulations on reduction in body weight, tumor volume, packed cell volume, percentage increase in life span (%ILS) and haematological parameters were compared. The data revealed were suggested that all the three Triphala formulation possessed significant anticancer activity and Triphala 1:2:3 formulation at 600 mg/kg b. w., possessed maximum anticancer activity among the 9 groups compared. Key words: Triphala, Antitumor activity; Ehrlich ascites carcinoma.

In the present study, methanolic extract of Triphala (an Ayurvedic formulation) and its constituents; Terminalia chebula, Terminalia Belerica & Emblica officinalis were evaluated & compared for the antioxidant activity. Antioxidant activity of the methanolic extracts were measured using ABTS & DPPH radical scavenging assays. The extracts were found to scavenge free radicals; ABTS & DPPH in a dose dependent manner. Total Phenolic content of the extracts were determined and expressed in terms of Gallic acid equivalents. On the basis of the results, it could be concluded that triphala and its constituents are potential source of natural antioxidants and the antioxidant activity is directly related to the phenolic compounds, suggesting direct contribution of phenolic compounds to these activities. Key words: Triphala, Emblica officinalis, Terminalia chebula, Terminalia belerica.